PUBLICATION
Anti-angiogenic activity of para-coumaric acid methyl ester on HUVECs in vitro and zebrafish in vivo
- Authors
- Zhang, H.Z., Li, C.Y., Wu, J.Q., Wang, R.X., Wei, P., Liu, M.H., He, M.F.
- ID
- ZDB-PUB-180910-2
- Date
- 2018
- Source
- Phytomedicine : international journal of phytotherapy and phytopharmacology 48: 10-20 (Journal)
- Registered Authors
- Keywords
- Anti-angiogenic activity, HUVEC, Xenograft, Zebrafish, pCAME
- MeSH Terms
-
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Coumaric Acids/pharmacology*
- Human Umbilical Vein Endothelial Cells/drug effects*
- Human Umbilical Vein Endothelial Cells/metabolism
- Humans
- Neovascularization, Pathologic/drug therapy*
- Receptors, TIE/metabolism
- Signal Transduction/drug effects*
- Vascular Endothelial Growth Factor A/metabolism
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 30195867 Full text @ Phytomedicine
Citation
Zhang, H.Z., Li, C.Y., Wu, J.Q., Wang, R.X., Wei, P., Liu, M.H., He, M.F. (2018) Anti-angiogenic activity of para-coumaric acid methyl ester on HUVECs in vitro and zebrafish in vivo. Phytomedicine : international journal of phytotherapy and phytopharmacology. 48:10-20.
Abstract
Background Para-coumaric acid methyl ester (pCAME) is one of the bioactive components of Costus speciosus (Koen) Sm. (Zingiberaceae). This plant is traditionally used in Asia to treat catarrhal fevers, worms, dyspepsia, and skin diseases.
Purpose To investigate the anti-angiogenic activity of pCAME and its molecular mechanism of action.
Study design We investigated the anti-angiogenic activity of pCAME on human umbilical vein endothelial cells (HUVECs) in vitro and zebrafish (Danio rerio) in vivo.
Methods In vitro cell proliferation, would healing, migration and tube formation assays were used, along with in vivo physiological angiogenic vessel formation, tumor-induced angiogenic vessel formation assays on zebrafish model. qRT-PCR and RNA-seq were also used for the target investigation.
Results pCAME could inhibit the proliferation, would healing, migration and tube formation of HUVECs, disrupt the physiological formation of intersegmental vessels (ISVs) and the subintestinal vessels (SIVs) of zebrafish embryos, and inhibit tumor angiogenesis in the zebrafish cell-line derived xenograft (zCDX) model of SGC-7901 in a dose-dependent manner. Mechanistic studies revealed that pCAME inhibited vegf/vegfr2 and ang/tie signaling pathways in zebrafish by quantitative RT-PCR analysis, and regulated multi-signaling pathways involving immune, inflammation and angiogenesis in SGC-7901 zCDX model by RNA-seq analysis.
Conclusion pCAME may be a multi-target anti-angiogenic drug candidate and hold great potential for developing novel therapeutic strategy for cancer treatment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping