PUBLICATION
Analysis of the Atlantic salmon genome reveals a cluster of Mx genes that respond more strongly to IFN gamma than to type I IFN
- Authors
- Robertsen, B., Greiner Tollersrud, L., Jørgensen, L.G.
- ID
- ZDB-PUB-180910-1
- Date
- 2018
- Source
- Developmental and comparative immunology 90: 80-89 (Journal)
- Registered Authors
- Keywords
- Antiviral, Evolution, Fish, Innate immunity, Interferon, Mx
- MeSH Terms
-
- Orthomyxoviridae Infections/immunology*
- Orthomyxoviridae Infections/virology
- Synteny/drug effects
- Multigene Family/genetics*
- Interferon Type I/metabolism
- Myxovirus Resistance Proteins/genetics*
- Myxovirus Resistance Proteins/metabolism
- Interferon-gamma/metabolism
- Animals
- Cell Line
- Phylogeny
- Antiviral Agents/metabolism
- Fish Diseases/immunology*
- Genome/genetics*
- Zebrafish
- Salmo salar/immunology*
- PubMed
- 30195710 Full text @ Dev. Comp. Immunol.
Citation
Robertsen, B., Greiner Tollersrud, L., Jørgensen, L.G. (2018) Analysis of the Atlantic salmon genome reveals a cluster of Mx genes that respond more strongly to IFN gamma than to type I IFN. Developmental and comparative immunology. 90:80-89.
Abstract
Mx proteins are antiviral GTPases, which are induced by type I IFN and virus infection. Analysis of the Atlantic salmon genome revealed the presence of 9 Mx genes localized to three chromosomes. A cluster of three Mx genes (SsaMx1 - SsaMx3), which includes previously cloned Mx genes, is present on chromosome (Chr) 12. A cluster of five Mx genes (SsaMx4-SsaMx8) is present on Chr25 while one Mx gene (SsaMx9) is present on Chr9. Phylogenetic and gene synteny analyses showed that SsaMx1-SsaMx3 are most closely related to the main group of teleost Mx proteins. In contrast, SsaMx 4-SsaMx9 formed a separate group together with zebrafish MxD and MxG and eel MxB. The Mx cluster in Chr25 showed gene synteny similar to a Mx gene cluster in the gar genome. Expression of Mx genes in cell lines stimulated with recombinant IFNs showed that Mx genes in Chr12 responded more strongly to type I IFN than to type II IFN (IFN gamma) whilst Mx genes in Chr25 responded more strongly to IFN gamma than to type I IFNs. SsaMx9 showed no response to the IFNs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping