PUBLICATION
phlda3 overexpression impairs specification of hemangioblasts and vascular development
- Authors
- Wang, X., Li, J., Yang, Z., Wang, L., Li, L., Deng, W., Zhou, J., Wang, L., Xu, C., Chen, Q., Wang, Q.K.
- ID
- ZDB-PUB-180907-11
- Date
- 2018
- Source
- The FEBS journal 285(21): 4071-4081 (Journal)
- Registered Authors
- Keywords
- AKT, phlda3, hemangioblast, hematopoiesis, vascular development
- MeSH Terms
-
- Animals
- Blood Vessels/embryology*
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/pathology*
- Gene Expression Regulation, Developmental
- Hemangioblasts/metabolism
- Hemangioblasts/pathology*
- Neovascularization, Pathologic*
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Proto-Oncogene Proteins c-akt/metabolism
- Signal Transduction
- Zebrafish/embryology*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30188605 Full text @ FEBS J.
Citation
Wang, X., Li, J., Yang, Z., Wang, L., Li, L., Deng, W., Zhou, J., Wang, L., Xu, C., Chen, Q., Wang, Q.K. (2018) phlda3 overexpression impairs specification of hemangioblasts and vascular development. The FEBS journal. 285(21):4071-4081.
Abstract
The phlda3 gene encodes a small, 127 amino acid protein with only a PH domain, and is involved in tumor suppression, proliferation of islet β-cells, insulin secretion, glucose tolerance, and liver injury. However, the role of phlda3 in vascular development is unknown. Here, we show that phlda3 overexpression decreases the expression levels of hemangioblast markers scl, fli1 and etsrp and intersegmental vessel (ISV) markers flk1 and cdh5, and disrupts ISV development in tg(flk1:GFP) and tg(fli1:GFP) zebrafish. Moreover, phlda3 overexpression inhibits the activation of AKT in zebrafish embryos, and the developmental defects of ISVs by phlda3 overexpression were reversed by the expression of a constitutively active form of AKT. These data suggest that phlda3 is a negative regulator of hemangioblast specification and ISV development via AKT signaling. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping