PUBLICATION
Tris(1,3-dichloro-2-propyl) phosphate Exposure During Early-Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis
- Authors
- Dasgupta, S., Cheng, V., Vliet, S.M.F., Mitchell, C.A., Volz, D.C.
- ID
- ZDB-PUB-180831-2
- Date
- 2018
- Source
- Environmental science & technology 52(18): 10820-10828 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastula
- Flame Retardants*
- Organophosphorus Compounds
- Phosphates
- Zebrafish*
- PubMed
- 30157643 Full text @ Env. Sci. Tech.
- CTD
- 30157643
Citation
Dasgupta, S., Cheng, V., Vliet, S.M.F., Mitchell, C.A., Volz, D.C. (2018) Tris(1,3-dichloro-2-propyl) phosphate Exposure During Early-Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis. Environmental science & technology. 52(18):10820-10828.
Abstract
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is an organophosphate flame retardant used around the world. Within zebrafish, we previously showed that initiation of TDCIPP exposure during cleavage (0.75 h post-fertilization, hpf) results in epiboly disruption at 6 hpf, leading to dorsalized embryos by 24 hpf - a phenotype that mimics the effects of dorsomorphin (DMP), a bone morphogenetic protein (BMP) antagonist that dorsalizes embryos in the absence of epiboly defects. The objective of this study was to 1) investigate the role of BMP signaling in TDCIPP-induced toxicity during early embryogenesis; 2) identify other pathways and processes targeted by TDCIPP; and 3) characterize downstream impacts of early developmental defects. Using zebrafish as a model, we first identified a sensitive window for TDCIPP-induced effects following exposure initiation at 0.75 hpf. We then investigated the effects of TDCIPP on the transcriptome during the first 24 h of development using mRNA- and amplicon-sequencing. Finally, we relied on whole-mount immunohistochemistry, dye-based labeling, and morphological assessments to study abnormalities later in embryonic development. Overall, our data suggest that initiation of TDCIPP exposure during early-blastula alters the normal trajectory of early embryogenesis by inducing gastrulation defects and aberrant germ layer formation, leading to abnormal tissue and organ development within the embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping