PUBLICATION
Multiscale light-sheet for rapid imaging of cardiopulmonary system
- Authors
- Ding, Y., Ma, J., Langenbacher, A.D., Baek, K.I., Lee, J., Chang, C.C., Hsu, J.J., Kulkarni, R.P., Belperio, J., Shi, W., Ranjbarvaziri, S., Ardehali, R., Tintut, Y., Demer, L.L., Chen, J.N., Fei, P., Packard, R.R.S., Hsiai, T.K.
- ID
- ZDB-PUB-180824-3
- Date
- 2018
- Source
- JCI insight 3(16): (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Newborn
- Embryo, Nonmammalian
- Heart/diagnostic imaging*
- Heart/embryology
- Heart/growth & development
- Imaging, Three-Dimensional/instrumentation
- Imaging, Three-Dimensional/methods*
- Intravital Microscopy/instrumentation
- Intravital Microscopy/methods*
- Light
- Microscopy, Fluorescence/instrumentation
- Microscopy, Fluorescence/methods
- Models, Animal
- Morphogenesis
- Respiratory System/diagnostic imaging*
- Respiratory System/embryology
- Respiratory System/growth & development
- Time-Lapse Imaging/instrumentation
- Time-Lapse Imaging/methods
- PubMed
- 30135307 Full text @ JCI Insight
Citation
Ding, Y., Ma, J., Langenbacher, A.D., Baek, K.I., Lee, J., Chang, C.C., Hsu, J.J., Kulkarni, R.P., Belperio, J., Shi, W., Ranjbarvaziri, S., Ardehali, R., Tintut, Y., Demer, L.L., Chen, J.N., Fei, P., Packard, R.R.S., Hsiai, T.K. (2018) Multiscale light-sheet for rapid imaging of cardiopulmonary system. JCI insight. 3(16):.
Abstract
The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping