PUBLICATION
Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males
- Authors
- Branco, G.S., Melo, A.G., Ricci, J.M.B., Digmayer, M., de Jesus, L.W.O., Habibi, H.R., Nóbrega, R.H.
- ID
- ZDB-PUB-180814-3
- Date
- 2018
- Source
- General and comparative endocrinology 273: 209-217 (Journal)
- Registered Authors
- Keywords
- Astyanax altiparanae, Follicle-stimulating hormone, Gonadotropin-inhibitory hormone, Gonadotropin-releasing hormone, Lambari-do-rabo-amarelo, Luteinizing hormone
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain/metabolism*
- Characidae/genetics
- Characidae/metabolism*
- Chickens
- Female
- Gonadotropin-Releasing Hormone/pharmacology*
- Male
- Models, Biological
- Neuropeptides/pharmacology*
- Pituitary Gland/anatomy & histology
- Pituitary Gland/drug effects
- Pituitary Gland/metabolism*
- RNA, Messenger/genetics
- Reference Standards
- Time Factors
- Zebrafish
- PubMed
- 30098316 Full text @ Gen. Comp. Endocrinol.
Citation
Branco, G.S., Melo, A.G., Ricci, J.M.B., Digmayer, M., de Jesus, L.W.O., Habibi, H.R., Nóbrega, R.H. (2018) Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males. General and comparative endocrinology. 273:209-217.
Abstract
In vertebrates, the pituitary gonadotropins Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone) control testicular development and function. In the pituitary, on the other hand, the synthesis and release of the gonadotropins relies on a complex regulatory system, which involves different signaling systems, such as GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone) neuropeptides. While GnRH exerts stimulatory roles on gonadotropin release and reproduction, the actions of GnIH remain incipient and controversial for many teleost species. Therefore, the aim of this study was to evaluate the effects of GnRH (chicken (c)GnRH2) and GnIH (zebrafish (z)GnIH-3) on the male gonadotropin expression using pituitaries explants of a neotropical species with economical and ecological relevance, Astyanax altiparanae. Moreover, the effects of these peptides on the GnRH expression were also analysed using male brain slices containing the hypothalamus and mid brain. Our results showed that in males cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on male basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression in A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping