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ZFIN ID: ZDB-PUB-180811-12
Construction, immune protection and innate immune response of shuffled polyvalent ompAs vaccines
Wang, S.N., Cheng, Z.X., Ling, X.P., Chu, X., Peng, X.X., Li, H.
Date: 2018
Source: Fish & shellfish immunology 74: 325-331 (Journal)
Registered Authors:
Keywords: DNA shuffling, E. tarda, Innate immune response, Polyvalent vaccine, V. alginolyticus, ompAs
MeSH Terms:
  • Animals
  • Bacterial Outer Membrane Proteins/genetics
  • Bacterial Outer Membrane Proteins/immunology*
  • Bacterial Proteins/genetics
  • Bacterial Proteins/immunology
  • Bacterial Vaccines/immunology*
  • DNA Shuffling/veterinary
  • Edwardsiella tarda/genetics
  • Edwardsiella tarda/immunology*
  • Enterobacteriaceae Infections/immunology
  • Enterobacteriaceae Infections/microbiology
  • Enterobacteriaceae Infections/veterinary
  • Escherichia coli/genetics
  • Escherichia coli/immunology
  • Escherichia coli Proteins/genetics
  • Escherichia coli Proteins/immunology
  • Fish Diseases/immunology*
  • Fish Diseases/microbiology
  • Recombinant Proteins/genetics
  • Recombinant Proteins/immunology
  • Vibrio Infections/immunology
  • Vibrio Infections/microbiology
  • Vibrio Infections/veterinary
  • Vibrio alginolyticus/genetics
  • Vibrio alginolyticus/immunology*
  • Vibrio parahaemolyticus/genetics
  • Vibrio parahaemolyticus/immunology
  • Zebrafish*
PubMed: 29289655 Full text @ Fish Shellfish Immunol.
Our previous studies demonstrated that molecular breeding via DNA shuffling directs the evolution of polyvalent vaccines with desired traits, which leads to generation of polyvalent ompA vaccines using Vibrio alginolyticus VA0764 primers. Here, we replaced VA0764 primers with Edwardsiella tarda ompA primers to generate new polyvalent ompA vaccines by DNA shuffling of the same five ompA genes from four species of bacteria E. tarda, V. parahaemolyticus, V. alginolyticus and Escherichia coli. We identified four polyvalent vaccine candidates from a eukaryotic expressing library EompAs-FE containing 82 ompAs using active immune protection against V. alginolyticus and E. tarda. Furthermore, we explored mechanisms of polyvalent vaccine candidates by investigation of the innate immune response to these ompAs, and found that expression of IL-1β, IL-8, IL-15, COX-2, IFN-γ, TLR-1, TLR-3 and C3b genes was elevated as a characteristic feature of these polyvalent vaccine candidates. These results indicate that use of different primers to construct a DNA library selects new evolution of polyvalent vaccines with desired traits, and polyvalent ompA vaccines elicit high innate immune response.