PUBLICATION

Construction, immune protection and innate immune response of shuffled polyvalent ompAs vaccines

Authors
Wang, S.N., Cheng, Z.X., Ling, X.P., Chu, X., Peng, X.X., Li, H.
ID
ZDB-PUB-180811-12
Date
2018
Source
Fish & shellfish immunology   74: 325-331 (Journal)
Registered Authors
Keywords
DNA shuffling, E. tarda, Innate immune response, Polyvalent vaccine, V. alginolyticus, ompAs
MeSH Terms
  • Animals
  • Escherichia coli Proteins/genetics
  • Escherichia coli Proteins/immunology
  • Zebrafish*
  • Bacterial Proteins/genetics
  • Bacterial Proteins/immunology
  • Vibrio parahaemolyticus/genetics
  • Vibrio parahaemolyticus/immunology
  • Vibrio alginolyticus/genetics
  • Vibrio alginolyticus/immunology*
  • Edwardsiella tarda/genetics
  • Edwardsiella tarda/immunology*
  • Bacterial Outer Membrane Proteins/genetics
  • Bacterial Outer Membrane Proteins/immunology*
  • Bacterial Vaccines/immunology*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/immunology
  • Escherichia coli/genetics
  • Escherichia coli/immunology
  • Vibrio Infections/immunology
  • Vibrio Infections/microbiology
  • Vibrio Infections/veterinary
  • Enterobacteriaceae Infections/immunology
  • Enterobacteriaceae Infections/microbiology
  • Enterobacteriaceae Infections/veterinary
  • Fish Diseases/immunology*
  • Fish Diseases/microbiology
  • DNA Shuffling/veterinary
PubMed
29289655 Full text @ Fish Shellfish Immunol.
Abstract
Our previous studies demonstrated that molecular breeding via DNA shuffling directs the evolution of polyvalent vaccines with desired traits, which leads to generation of polyvalent ompA vaccines using Vibrio alginolyticus VA0764 primers. Here, we replaced VA0764 primers with Edwardsiella tarda ompA primers to generate new polyvalent ompA vaccines by DNA shuffling of the same five ompA genes from four species of bacteria E. tarda, V. parahaemolyticus, V. alginolyticus and Escherichia coli. We identified four polyvalent vaccine candidates from a eukaryotic expressing library EompAs-FE containing 82 ompAs using active immune protection against V. alginolyticus and E. tarda. Furthermore, we explored mechanisms of polyvalent vaccine candidates by investigation of the innate immune response to these ompAs, and found that expression of IL-1?, IL-8, IL-15, COX-2, IFN-?, TLR-1, TLR-3 and C3b genes was elevated as a characteristic feature of these polyvalent vaccine candidates. These results indicate that use of different primers to construct a DNA library selects new evolution of polyvalent vaccines with desired traits, and polyvalent ompA vaccines elicit high innate immune response.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping