PUBLICATION
The protective efficacy of four iron-related recombinant proteins and their single-walled carbon nanotube encapsulated counterparts against Aeromonas hydrophila infection in zebrafish
- Authors
- Guo, Z., Lin, Y., Wang, X., Fu, Y., Lin, W., Lin, X.
- ID
- ZDB-PUB-180808-19
- Date
- 2018
- Source
- Fish & shellfish immunology 82: 50-59 (Journal)
- Registered Authors
- Keywords
- Aeromonas hydrophila, Immunoprotective effect, Iron homeostasis, Single-walled carbon nanotube
- MeSH Terms
-
- Aeromonas hydrophila/physiology
- Animals
- Bacterial Proteins/pharmacology*
- Fish Diseases/immunology*
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/veterinary*
- Immunity, Innate*
- Injections, Intraperitoneal/veterinary
- Iron
- Nanotubes, Carbon/analysis*
- Random Allocation
- Recombinant Proteins/pharmacology
- Zebrafish*
- PubMed
- 30086377 Full text @ Fish Shellfish Immunol.
Citation
Guo, Z., Lin, Y., Wang, X., Fu, Y., Lin, W., Lin, X. (2018) The protective efficacy of four iron-related recombinant proteins and their single-walled carbon nanotube encapsulated counterparts against Aeromonas hydrophila infection in zebrafish. Fish & shellfish immunology. 82:50-59.
Abstract
Iron-related proteins play important roles in iron homeostasis, and they may be potential vaccine candidates against pathogenic Aeromonas hydrophila. In addition, the encapsulation of antigens in single-walled carbon nanotubes (SWCNTs) has recently been shown to effectively stimulate the host immune response. To investigate the immune response of zebrafish to iron-related proteins and SWCNT-encapsulated proteins, we overexpressed and purified four iron-related recombinant proteins (P55870, A0KGK5, A0KPP0, and A0KIY3) from A. hydrophila. We then vaccinated zebrafish with these proteins and their SWCNT-encapsulated counterparts via both intraperitoneal injection and bath immunization. The target proteins evoked an immune response in zebrafish after intraperitoneal injection, and SWCNT-encapsulation significantly increased the immune response after bath immunization. When challenged with virulent A. hydrophila, zebrafish administered 5 μg intraperitoneal injections of SWCNT-P55870, A0KGK5, A0KPP0, or A0KIY3 had remarkably high relative percent survivals (RPSs) (50%, 55.6%, 66.7%, and 94.44% respectively). The RPSs of zebrafish vaccinated via immunization bath with 40 mg/L SWCNT-encapsulated counterparts were also high (52.94%, 55.56%, 61.11%, and 86.11%, respectively). These results indicated that zebrafish vaccinated with P55870, A0KGK5, SWCNT-P55870, and SWCNT-A0KGK5 were partially protected, while A0KPP0 and A0KIY3 were promising vaccine candidates against pathogenic A. hydrophila infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping