PUBLICATION
Dysbiosis and Early Mortality in Zebrafish Larvae Exposed to Subclinical Concentrations of Streptomycin
- Authors
- Pindling, S., Azulai, D., Zheng, B., Dahan, D., Perron, G.G.
- ID
- ZDB-PUB-180808-13
- Date
- 2018
- Source
- FEMS microbiology letters 365(18): (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/administration & dosage*
- Drug Resistance, Bacterial
- Dysbiosis/chemically induced*
- Gastrointestinal Microbiome/drug effects*
- Gene Transfer, Horizontal
- Interspersed Repetitive Sequences
- Larva/drug effects*
- Larva/microbiology
- Larva/physiology
- Selection, Genetic
- Streptomycin/administration & dosage*
- Survival Analysis
- Zebrafish/embryology*
- Zebrafish/microbiology
- PubMed
- 30085054 Full text @ FEMS Microbiol. Lett.
Citation
Pindling, S., Azulai, D., Zheng, B., Dahan, D., Perron, G.G. (2018) Dysbiosis and Early Mortality in Zebrafish Larvae Exposed to Subclinical Concentrations of Streptomycin. FEMS microbiology letters. 365(18).
Abstract
Exposure to low concentrations of antibiotics found in aquatic environments can increase susceptibility to infection in adult fish due microbiome disruption. However, little is known regarding the effect of antibiotic pollution on fish larvae. Here, we show that exposure to streptomycin, a common antibiotic used in medicine and aquaculture, disrupts the normal composition of zebrafish larvae microbiomes, significantly reducing the microbial diversity found in the fish. Exposure to streptomycin also significantly increased early mortality among fish larvae, causing full mortality within a few days of exposure at 10 μg/mL. Finally, we found that subclinical concentrations of streptomycin also increased the abundance of class 1 integrons, an integrase-dependent genetic system associated to the horizontal transfer of antibiotic resistance genes, in the larvae microbiomes. These results suggest that even low concentrations of streptomycin associated with environmental pollution could impact on fish populations and lead to the creation of antibiotic resistance reservoirs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping