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ZFIN ID: ZDB-PUB-180803-8
Macrophages inhibit Aspergillus fumigatus germination and neutrophil-mediated fungal killing
Rosowski, E.E., Raffa, N., Knox, B.P., Golenberg, N., Keller, N.P., Huttenlocher, A.
Date: 2018
Source: PLoS pathogens   14: e1007229 (Journal)
Registered Authors: Huttenlocher, Anna, Rosowski, Emily E.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aspergillosis/immunology
  • Aspergillosis/microbiology
  • Aspergillus fumigatus/genetics
  • Aspergillus fumigatus/immunology*
  • Aspergillus fumigatus/physiology*
  • Cytotoxicity, Immunologic*
  • Larva
  • Macrophages/physiology*
  • Neutrophils/physiology*
  • Organisms, Genetically Modified
  • Phagocytosis/immunology
  • Spores, Fungal/genetics
  • Spores, Fungal/immunology*
  • Zebrafish/embryology
  • Zebrafish/growth & development
  • Zebrafish/immunology
PubMed: 30071103 Full text @ PLoS Pathog.
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ABSTRACT
In immunocompromised individuals, Aspergillus fumigatus causes invasive fungal disease that is often difficult to treat. Exactly how immune mechanisms control A. fumigatus in immunocompetent individuals remains unclear. Here, we use transparent zebrafish larvae to visualize and quantify neutrophil and macrophage behaviors in response to different A. fumigatus strains. We find that macrophages form dense clusters around spores, establishing a protective niche for fungal survival. Macrophages exert these protective effects by inhibiting fungal germination, thereby inhibiting subsequent neutrophil recruitment and neutrophil-mediated killing. Germination directly drives fungal clearance as faster-growing CEA10-derived strains are killed better in vivo than slower-growing Af293-derived strains. Additionally, a CEA10 pyrG-deficient strain with impaired germination is cleared less effectively by neutrophils. Host inflammatory activation through Myd88 is required for killing of a CEA10-derived strain but not sufficient for killing of an Af293-derived strain, further demonstrating the role of fungal-intrinsic differences in the ability of a host to clear an infection. Altogether, we describe a new role for macrophages in the persistence of A. fumigatus and highlight the ability of different A. fumigatus strains to adopt diverse modes of virulence.
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