PUBLICATION
Scale-invariant patterning by size-dependent inhibition of Nodal signalling
- Authors
- Almuedo-Castillo, M., Bläßle, A., Mörsdorf, D., Marcon, L., Soh, G.H., Rogers, K.W., Schier, A.F., Müller, P.
- ID
- ZDB-PUB-180801-8
- Date
- 2018
- Source
- Nature cell biology 20(9): 1032-1042 (Journal)
- Registered Authors
- Müller, Patrick, Rogers, Katherine, Schier, Alexander
- Keywords
- none
- MeSH Terms
-
- Body Patterning*/genetics
- Animals
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Signal Transduction
- Time Factors
- Animals, Genetically Modified
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Gene Expression Regulation, Developmental
- Left-Right Determination Factors/genetics
- Left-Right Determination Factors/metabolism*
- Blastula/metabolism*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- PubMed
- 30061678 Full text @ Nat. Cell Biol.
Citation
Almuedo-Castillo, M., Bläßle, A., Mörsdorf, D., Marcon, L., Soh, G.H., Rogers, K.W., Schier, A.F., Müller, P. (2018) Scale-invariant patterning by size-dependent inhibition of Nodal signalling. Nature cell biology. 20(9):1032-1042.
Abstract
Individuals can vary substantially in size, but the proportions of their body plans are often maintained. We generated smaller zebrafish by removing 30% of their cells at the blastula stages and found that these embryos developed into normally patterned individuals. Strikingly, the proportions of all germ layers adjusted to the new embryo size within 2?hours after cell removal. As Nodal-Lefty signalling controls germ-layer patterning, we performed a computational screen for scale-invariant models of this activator-inhibitor system. This analysis predicted that the concentration of the highly diffusive inhibitor Lefty increases in smaller embryos, leading to a decreased Nodal activity range and contracted germ-layer dimensions. In vivo studies confirmed that Lefty concentration increased in smaller embryos, and embryos with reduced Lefty levels or with diffusion-hindered Lefty failed to scale their tissue proportions. These results reveal that size-dependent inhibition of Nodal signalling allows scale-invariant patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping