PUBLICATION

Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish

Authors
Hanovice, N.J., McMains, E., Gross, J.M.
ID
ZDB-PUB-180801-12
Date
2018
Source
Methods in molecular biology (Clifton, N.J.)   1821: 359-370 (Chapter)
Registered Authors
Gross, Jeffrey, Hanovice, Nick
Keywords
Cdc42, GAL4/UAS, Heat shock, Rac1, RhoA, Transgenics, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified*/embryology
  • Animals, Genetically Modified*/genetics
  • DNA-Binding Proteins*/genetics
  • DNA-Binding Proteins*/metabolism
  • Embryo, Nonmammalian/embryology*
  • Embryonic Development*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Transcription Factors*/genetics
  • Transcription Factors*/metabolism
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • rho GTP-Binding Proteins*/biosynthesis
  • rho GTP-Binding Proteins*/genetics
PubMed
30062424 Full text @ Meth. Mol. Biol.
Abstract
Rho GTPases are Ras-family G proteins that regulate many critical cellular functions. Due to their requirement during early embryonic development, investigations into the function of Rho GTPases at a tissue-specific level require inducible and spatially targeted modulation of Rho GTPase activity. Here, we describe the use of ten novel zebrafish transgenics enabling GAL4-specific expression of Rho GTPases to modulate Rho GTPase activity with spatial and temporal control.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping