ZFIN ID: ZDB-PUB-180722-24
Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner
Espina, J., Marchant, C., De Ferrari, G.V., Reyes, A.E.
Date: 2018
Source: Mechanisms of Development   154: 203-207 (Journal)
Registered Authors: Espina, Jaime
Keywords: none
MeSH Terms:
  • Animals
  • Cell Movement/genetics*
  • Epithelial-Mesenchymal Transition/genetics
  • Hypoxia-Inducible Factor 1, alpha Subunit/genetics*
  • Neural Crest/growth & development*
  • Organogenesis/genetics
  • Oxygen/metabolism*
  • Xenopus laevis/genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 30031069 Full text @ Mech. Dev.
The transcription factor Hif-1α regulates epithelial to mesenchymal transition and neural crest cell chemotaxis in Xenopus. Hif-1α is only stabilised under low oxygen levels, and the in vivo stabilisation of this factor in neural crest cells is poorly understood. Multiple oxygen-independent Hif-1α regulators have been described in cell cultures and cancer models. Among these, the PDGF pathway has been linked to neural crest development. The present study established a connection between the Pdgf pathway and Hif-1α stabilisation in zebrafish. Specifically, embryos with a disrupted Pdgf pathway were rescued by employing hif-1α mRNA through qPCR and immunohistochemistry techniques. The data suggest that oxygen levels in the neural crest are normal and that Pdgf1aa regulates neural crest migration through Hif-1α expression.