PUBLICATION
Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner
- Authors
- Espina, J., Marchant, C., De Ferrari, G.V., Reyes, A.E.
- ID
- ZDB-PUB-180722-24
- Date
- 2018
- Source
- Mechanisms of Development 154: 203-207 (Journal)
- Registered Authors
- Espina, Jaime
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement/genetics*
- Epithelial-Mesenchymal Transition/genetics
- Hypoxia-Inducible Factor 1, alpha Subunit/genetics*
- Neural Crest/growth & development*
- Organogenesis/genetics
- Oxygen/metabolism*
- Xenopus laevis/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 30031069 Full text @ Mech. Dev.
Citation
Espina, J., Marchant, C., De Ferrari, G.V., Reyes, A.E. (2018) Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner. Mechanisms of Development. 154:203-207.
Abstract
The transcription factor Hif-1α regulates epithelial to mesenchymal transition and neural crest cell chemotaxis in Xenopus. Hif-1α is only stabilised under low oxygen levels, and the in vivo stabilisation of this factor in neural crest cells is poorly understood. Multiple oxygen-independent Hif-1α regulators have been described in cell cultures and cancer models. Among these, the PDGF pathway has been linked to neural crest development. The present study established a connection between the Pdgf pathway and Hif-1α stabilisation in zebrafish. Specifically, embryos with a disrupted Pdgf pathway were rescued by employing hif-1α mRNA through qPCR and immunohistochemistry techniques. The data suggest that oxygen levels in the neural crest are normal and that Pdgf1aa regulates neural crest migration through Hif-1α expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping