PUBLICATION
The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos.
- Authors
- Della Torre, C., Maggioni, D., Ghilardi, A., Parolini, M., Santo, N., Landi, C., Madaschi, L., Magni, S., Tasselli, S., Ascagni, M., Bini, L., La Porta, C., Del Giacco, L., Binelli, A.
- ID
- ZDB-PUB-180722-13
- Date
- 2018
- Source
- Environmental pollution (Barking, Essex : 1987) 241: 999-1008 (Journal)
- Registered Authors
- Del Giacco, Luca
- Keywords
- Danio rerio, Fullerene nanoparticles, Oxidative stress, Proteomics, Trojan horse effect
- MeSH Terms
-
- Animals
- Benzo(a)pyrene/chemistry
- Benzo(a)pyrene/toxicity*
- Biological Availability
- DNA Damage/drug effects
- Fullerenes/chemistry
- Fullerenes/toxicity*
- Nanoparticles
- Oxidative Stress/drug effects
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 30029334 Full text @ Environ. Pollut.
Citation
Della Torre, C., Maggioni, D., Ghilardi, A., Parolini, M., Santo, N., Landi, C., Madaschi, L., Magni, S., Tasselli, S., Ascagni, M., Bini, L., La Porta, C., Del Giacco, L., Binelli, A. (2018) The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos.. Environmental pollution (Barking, Essex : 1987). 241:999-1008.
Abstract
This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles (C60) and Benzo(α)pyrene (B(α)P) on zebrafish embryos, which were exposed to C60 and B(α)P alone and to C60 doped with B(α)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C60 interaction with B(α)P. The carrier role of C60 for B(α)P was observed, however adsorption on C60 did not affect the accumulation and localization of B(α)P in the embryos. Instead, C60 doped with B(α)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C60 alone. As for toxicity, our results suggested that C60 alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C60 + B(α)P induced cellular response mechanisms similar to B(α)P alone, but generating greater cellular damages in the exposed embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping