PUBLICATION
Eco-friendly profile of pegylated nano-graphene oxide at different levels of an aquatic trophic chain
- Authors
- Loureiro, S., Gonçalves, S.F., Gonçalves, G., Hortiguela, M.J., Rebelo, S., Ferro, M.C., Vila, M.
- ID
- ZDB-PUB-180711-7
- Date
- 2018
- Source
- Ecotoxicology and environmental safety 162: 192-200 (Journal)
- Registered Authors
- Loureiro, Susana
- Keywords
- Algae, Daphnia, Fish, Radiation, nGO-PEG
- MeSH Terms
-
- Animals
- Antineoplastic Agents
- Chlorophyta/drug effects
- Chlorophyta/growth & development
- Daphnia/drug effects
- Drug Delivery Systems
- Embryo, Nonmammalian/drug effects
- Food Chain
- Graphite/chemistry
- Graphite/toxicity*
- Oxides/chemistry
- Oxides/toxicity*
- Polyethylene Glycols/chemistry
- Polyethylene Glycols/toxicity*
- Toxicity Tests/methods
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- PubMed
- 29990731 Full text @ Ecotoxicol. Environ. Saf.
Citation
Loureiro, S., Gonçalves, S.F., Gonçalves, G., Hortiguela, M.J., Rebelo, S., Ferro, M.C., Vila, M. (2018) Eco-friendly profile of pegylated nano-graphene oxide at different levels of an aquatic trophic chain. Ecotoxicology and environmental safety. 162:192-200.
Abstract
Nanographene oxide (nGO) has been recently proposed as a new antitumoral therapeutic agent, drug delivery carrier and gene transfection vehicle, among others. Treatment is carried out by hyperthermia induced by infrared irradiation. After treatment, the nanosystems will be inevitably excreted and released to the environment. To understand the potential impacts of pegylated nGO (nGO-PEG), three key species from different trophic levels were used: the green micro-algae Raphidocelis subcapitata (growth inhibition test), the cladocera Daphnia magna (acute and chronic tests), and the fish Danio rerio (fish embryo test). Besides a regular standard procedure to assess toxicity, and considering the mode of action of nGO-PEG in cancer treatment, a simultaneous infrared lamp exposure was carried out for D. magna and D. rerio. Additionally, and taking advantage of the phenotypic transparency of D. magna, nGO-PEG was fluorescently tagged to evaluate the potential uptake of nGO-PEG. The R. subcapitata growth inhibition test showed effects during the first 48 h, recovering till the end of the test (96 h). No acute or chronic effects were observed for D. magna, under standard or infrared light exposures although confocal microscope images showed nGO-PEG uptake. Very small percentages of mortality and abnormalities were observed in D. rerio exposed with and without the infrared lamp. Although low hazard may be expected for nGO-PEG in aquatic ecosystems, further studies with species with different life traits should be accomplished, in order to derive more accurate conclusions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping