PUBLICATION
Spatial Fold Change of FGF Signaling Encodes Positional Information for Segmental Determination in Zebrafish
- Authors
- Simsek, M.F., Özbudak, E.M.
- ID
- ZDB-PUB-180705-5
- Date
- 2018
- Source
- Cell Reports 24: 66-78.e8 (Journal)
- Registered Authors
- Ozbudak, Ertugrul, Simsek, Muhammed
- Keywords
- none
- MeSH Terms
-
- Fibroblast Growth Factors/metabolism*
- Models, Biological
- Signal Transduction*
- Animals
- Mosaicism
- Body Patterning*
- Somites/embryology
- Wnt Proteins/metabolism
- Embryo, Nonmammalian/metabolism
- Tail
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 29972792 Full text @ Cell Rep.
Citation
Simsek, M.F., Özbudak, E.M. (2018) Spatial Fold Change of FGF Signaling Encodes Positional Information for Segmental Determination in Zebrafish. Cell Reports. 24:66-78.e8.
Abstract
Signal gradients encode instructive information for numerous decision-making processes during embryonic development. A striking example of precise, scalable tissue-level patterning is the segmentation of somites-the precursors of the vertebral column-during which the fibroblast growth factor (FGF), Wnt, and retinoic acid (RA) pathways establish spatial gradients. Despite decades of studies proposing roles for all three pathways, the dynamic feature of these gradients that encodes instructive information determining segment sizes remained elusive. We developed a non-elongating tail explant system, integrated quantitative measurements with computational modeling, and tested alternative models to show that positional information is encoded solely by spatial fold change (SFC) in FGF signal output. Neighboring cells measure SFC to accurately position the determination front and thus determine segment size. The SFC model successfully recapitulates results of spatiotemporal perturbation experiments on both explants and intact embryos, and it shows that Wnt signaling acts permissively upstream of FGF signaling and that RA gradient is dispensable.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping