PUBLICATION
Transcriptional effects of androstenedione and 17α-hydroxyprogesterone in zebrafish embryos
- Authors
- Fent, K., Siegenthaler, P.F., Schmid, A.A.
- ID
- ZDB-PUB-180701-7
- Date
- 2018
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 202: 1-5 (Journal)
- Registered Authors
- Keywords
- 17α-hydroxyprogesterone, Androgen biomarker, Androsentedione, Testosterone, Transcription analysis, Zebrafish
- MeSH Terms
-
- Androstenedione/toxicity*
- Animals
- Aromatase/genetics
- Aromatase/metabolism
- Cluster Analysis
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Progesterone/analogs & derivatives*
- Progesterone/toxicity
- Receptors, Progesterone/genetics
- Receptors, Progesterone/metabolism
- Sulfotransferases/genetics
- Sulfotransferases/metabolism
- Transcription, Genetic/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29960009 Full text @ Aquat. Toxicol.
Citation
Fent, K., Siegenthaler, P.F., Schmid, A.A. (2018) Transcriptional effects of androstenedione and 17α-hydroxyprogesterone in zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 202:1-5.
Abstract
Steroid hormones in the aquatic environment may pose a risk to fish health. Here we evaluated effects of two different class steroids that frequently occur in the aquatic environment, the androgen androstenedione (A4) and the progestin 17α-hydroxyprogesterone (17-OHP4). Zebrafish embryos were exposed to four concentrations of A4 and the positive control testosterone and to 17-OHP4, and transcriptional changes were determined at 96 h post fertilization (hpf) and 120 hpf. Transcriptional changes of 18 selected genes were assessed upon exposure to measured concentrations of 0.004, 0.046, 0.62 and 6.56 μg/L A4. Significant induction of the genes encoding sulfotransferase (sult2st3) and aromatase (cyp19b) occurred in 120 hpf embryos at 6.56 μg/L A4 and 1 μg/L testosterone. Additionally, cyp2k7 was significantly induced in two of three independent experiments. 17-OHP4 did not induce physiological effects (muscle contraction, heart rate, hatching success, swimming activity) at concentrations between 0.01 and 10 μg/L. Of the analyzed 15 genes, slight transcriptional alterations occurred for the genes encoding progesterone receptor, aromatases (cyp19a) and (cyp19b) and cyp2k7 at 10 μg/L. Our study highlights sult2st3, cyp19b and cyp2k7 as potential markers of androgen exposure in fish and indicates that 17-OHP4 is not likely to pose a risk for fish at environmental concentrations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping