PUBLICATION
Transcriptional effects of androstenedione and 17?-hydroxyprogesterone in zebrafish embryos
- Authors
- Fent, K., Siegenthaler, P.F., Schmid, A.A.
- ID
- ZDB-PUB-180701-7
- Date
- 2018
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 202: 1-5 (Journal)
- Registered Authors
- Keywords
- 17?-hydroxyprogesterone, Androgen biomarker, Androsentedione, Testosterone, Transcription analysis, Zebrafish
- MeSH Terms
-
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Cluster Analysis
- Aromatase/genetics
- Aromatase/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Androstenedione/toxicity*
- Transcription, Genetic/drug effects*
- Progesterone/analogs & derivatives*
- Progesterone/toxicity
- Sulfotransferases/genetics
- Sulfotransferases/metabolism
- Animals
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Receptors, Progesterone/genetics
- Receptors, Progesterone/metabolism
- PubMed
- 29960009 Full text @ Aquat. Toxicol.
Citation
Fent, K., Siegenthaler, P.F., Schmid, A.A. (2018) Transcriptional effects of androstenedione and 17?-hydroxyprogesterone in zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 202:1-5.
Abstract
Steroid hormones in the aquatic environment may pose a risk to fish health. Here we evaluated effects of two different class steroids that frequently occur in the aquatic environment, the androgen androstenedione (A4) and the progestin 17?-hydroxyprogesterone (17-OHP4). Zebrafish embryos were exposed to four concentrations of A4 and the positive control testosterone and to 17-OHP4, and transcriptional changes were determined at 96?h post fertilization (hpf) and 120 hpf. Transcriptional changes of 18 selected genes were assessed upon exposure to measured concentrations of 0.004, 0.046, 0.62 and 6.56 ?g/L A4. Significant induction of the genes encoding sulfotransferase (sult2st3) and aromatase (cyp19b) occurred in 120 hpf embryos at 6.56 ?g/L A4 and 1 ?g/L testosterone. Additionally, cyp2k7 was significantly induced in two of three independent experiments. 17-OHP4 did not induce physiological effects (muscle contraction, heart rate, hatching success, swimming activity) at concentrations between 0.01 and 10 ?g/L. Of the analyzed 15 genes, slight transcriptional alterations occurred for the genes encoding progesterone receptor, aromatases (cyp19a) and (cyp19b) and cyp2k7 at 10 ?g/L. Our study highlights sult2st3, cyp19b and cyp2k7 as potential markers of androgen exposure in fish and indicates that 17-OHP4 is not likely to pose a risk for fish at environmental concentrations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping