PUBLICATION
The Use of Chitosan-Coated Membrane Vesicles for Immunization Against Salmonid Rickettsial Septicemia in an Adult Zebrafish Model
- Authors
- Tandberg, J., Lagos, L., Ropstad, E., Smistad, G., Hiorth, M., Winther-Larsen, H.C.
- ID
- ZDB-PUB-180630-11
- Date
- 2018
- Source
- Zebrafish 15(4): 372-381 (Journal)
- Registered Authors
- Keywords
- MVs, Piscirickettsia salmonis, chitosan, immune response, vaccine, zebrafish
- MeSH Terms
-
- Chitosan/administration & dosage*
- Chitosan/chemistry
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Animals
- Immunity, Innate
- Disease Models, Animal
- Gene Expression Regulation/drug effects
- Immunization
- Sepsis/immunology
- Sepsis/microbiology
- Sepsis/prevention & control*
- Fish Diseases/immunology
- Fish Diseases/microbiology
- Fish Diseases/prevention & control*
- Adjuvants, Immunologic
- Zebrafish*
- Bacterial Vaccines/administration & dosage*
- Cytoplasmic Vesicles/chemistry*
- Piscirickettsiaceae Infections/immunology
- Piscirickettsiaceae Infections/microbiology
- Piscirickettsiaceae Infections/prevention & control*
- Piscirickettsia/immunology
- PubMed
- 29957152 Full text @ Zebrafish
Citation
Tandberg, J., Lagos, L., Ropstad, E., Smistad, G., Hiorth, M., Winther-Larsen, H.C. (2018) The Use of Chitosan-Coated Membrane Vesicles for Immunization Against Salmonid Rickettsial Septicemia in an Adult Zebrafish Model. Zebrafish. 15(4):372-381.
Abstract
The introduction of fish vaccination has had a tremendous impact on the aquaculture industry by providing an important measurement in regard to disease control. Infectious diseases caused by intracellular pathogens do, however, remain an unsolved problem for the industry. This is in many cases directly connected to the inability of vaccines to evoke a cellular immunity needed for long-term protection. Thus, there is a need for new and improved vaccines and adjuvants able to induce a strong humoral and cellular immune response. We have previously shown that membrane vesicles (MVs) from the intracellular fish pathogen Piscirickettsia salmonis are able to induce a protective response in adult zebrafish, but the incorporation of an adjuvant has not been evaluated. In this study, we report the use of chitosan as an adjuvant in combination with the P. salmonis-derived MVs for improved immunization against P. salmonis. Both free chitosan and chitosan-coated MVs (cMVs) were injected into adult zebrafish and their efficacy evaluated. The cMVs provided a significant protection (p?
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping