PUBLICATION
Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells
- Authors
- He, S., Chen, J., Jiang, Y., Wu, Y., Zhu, L., Jin, W., Zhao, C., Yu, T., Wang, T., Wu, S., Lin, X., Qu, J.Y., Wen, Z., Zhang, W., Xu, J.
- ID
- ZDB-PUB-180616-8
- Date
- 2018
- Source
- eLIFE 7: (Journal)
- Registered Authors
- Wen, Zilong, Zhang, Wenqing
- Keywords
- developmental biology, stem cells, zebrafish
- MeSH Terms
-
- Zebrafish
- Animals
- Mice
- Aorta/cytology
- Aorta/embryology
- Aorta/growth & development
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Gonads/cytology
- Gonads/embryology
- Gonads/growth & development
- Hematopoietic Stem Cells/cytology
- Hematopoietic Stem Cells/physiology*
- Langerhans Cells/cytology
- Langerhans Cells/physiology*
- Cell Lineage/physiology*
- Animals, Genetically Modified
- Macrophages/metabolism
- Mesonephros/cytology
- Mesonephros/embryology
- Mesonephros/growth & development
- Cell Differentiation/physiology*
- Hematopoietic Stem Cell Transplantation
- Microscopy, Confocal
- PubMed
- 29905527 Full text @ Elife
Citation
He, S., Chen, J., Jiang, Y., Wu, Y., Zhu, L., Jin, W., Zhao, C., Yu, T., Wang, T., Wu, S., Lin, X., Qu, J.Y., Wen, Z., Zhang, W., Xu, J. (2018) Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells. eLIFE. 7.
Abstract
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tracing method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult LCs originated from the ventral wall of the dorsal aorta (VDA), an equivalent to the mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem cells (HSCs) and non-HSC progenitors are generated. Further fine-fate mapping analysis revealed that the appearance of LCs in adult zebrafish was correlated with the development of HSCs, but not T cell progenitors. Finally, we showed that the appearance of tissue-resident macrophages in the brain, liver, heart, and gut of adult zebrafish was also correlated with HSCs. Thus, the results of our study challenged the EMP-origin theory for LCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping