PUBLICATION

Toxicity and non-harmful effects of the soya isoflavones, genistein and daidzein, in embryos of the zebrafish, Danio rerio

Authors
Sarasquete, C., Úbeda-Manzanaro, M., Ortiz-Delgado, J.B.
ID
ZDB-PUB-180606-14
Date
2018
Source
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   211: 57-67 (Journal)
Registered Authors
Keywords
Apoptosis, Daidzein, Embryos, Genistein, Larvae, Thyroid, Toxicity, Zebrafish, cyp1a, esrrb
MeSH Terms
  • Gene Expression Regulation, Developmental*
  • Thyroid Gland/embryology
  • Thyroid Gland/enzymology
  • Thyroid Gland/metabolism*
  • Receptors, Estrogen/chemistry
  • Receptors, Estrogen/genetics
  • Receptors, Estrogen/metabolism
  • Signal Transduction
  • fas Receptor/agonists
  • fas Receptor/chemistry
  • fas Receptor/metabolism
  • Toxicity Tests, Acute
  • Ectogenesis
  • Genistein/adverse effects*
  • Genistein/metabolism
  • Seeds/chemistry
  • Lethal Dose 50
  • Isoflavones/adverse effects*
  • Isoflavones/metabolism
  • Glycine max/chemistry
  • Embryo, Nonmammalian/enzymology
  • Embryo, Nonmammalian/metabolism*
  • Animals
  • Apoptosis
  • Endocrine Disruptors/adverse effects
  • Endocrine Disruptors/metabolism
  • Cytochrome P-450 CYP1A1/chemistry
  • Cytochrome P-450 CYP1A1/genetics
  • Cytochrome P-450 CYP1A1/metabolism
  • Dietary Supplements/adverse effects
  • Larva/enzymology
  • Larva/growth & development
  • Larva/metabolism
  • Phytoestrogens/adverse effects*
  • Zebrafish
(all 35)
PubMed
29870789 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Abstract
Based on the assumed oestrogenic and apoptotic properties of soya isoflavones (genistein, daidzein), and following the current OECD test-guidelines and principle of 3Rs, we have studied the potential toxicity of phytochemicals on the zebrafish embryos test (ZFET). For this purpose, zebrafish embryos at 2-3?h post-fertilisation (hpf) were exposed to both soya isoflavones (from 1.25?mg/L to 20?mg/L) and assayed until 96?hpf. Lethal and sub-lethal endpoints (mortality, hatching rates and malformations) were estimated in the ZFET, which was expanded to potential gene expression markers, determining the lowest observed effect (and transcriptional) concentrations (LOEC, LOTEC), and the no-observable effect (and transcriptional) concentrations (NOEC, NOTEC). The results revealed that genistein is more toxic (LC50-96?hpf: 4.41?mg/L) than daidzein (over 65.15?mg/L). Both isoflavones up-regulated the oestrogen (esrrb) and death receptors (fas) and cyp1a transcript levels. Most thyroid transcript signals were up-regulated by genistein (except for thyroid peroxidase/tpo), and the hatching enzyme (he1a1) was exclusively up-regulated by daidzein (from 1.25?mg/L onwards). The ZFET proved suitable for assessing toxicant effects of both isoflavones and potential disruptions (i.e. oestrogenic, apoptotic, thyroid, enzymatic) during the embryogenesis and the endotrophic larval period.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Sarasquete, C., Úbeda-Manzanaro, M., Ortiz-Delgado, J.B. (2018) Toxicity and non-harmful effects of the soya isoflavones, genistein and daidzein, in embryos of the zebrafish, Danio rerio. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 211:57-67.