PUBLICATION
A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity
- Authors
- Qu, L., Zhao, C., Wang, C., Gu, S., Zhang, M., Deng, X., Song, W.
- ID
- ZDB-PUB-180531-13
- Date
- 2018
- Source
- Bulletin of environmental contamination and toxicology 101(1): 80-85 (Journal)
- Registered Authors
- Keywords
- Acute toxicity, Angiogenesis, Cardiotoxicity, Musk ambrette, Neurotoxicity, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biological Assay
- Dinitrobenzenes/toxicity*
- Embryo, Nonmammalian/drug effects
- Lethal Dose 50
- Mutagens/toxicity*
- Tandem Mass Spectrometry
- Toxicity Tests, Acute*
- Zebrafish*
- PubMed
- 29846757 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Qu, L., Zhao, C., Wang, C., Gu, S., Zhang, M., Deng, X., Song, W. (2018) A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity. Bulletin of environmental contamination and toxicology. 101(1):80-85.
Abstract
Musk ambrette (4-tert-butyl-3-methoxy-2,6-dinitrotoluene) is a nitro musk, a cheap substitute for natural musk and a potential environmental pollutant based on its persistence, accumulation in human organisms. We investigated the acute toxicity of musk ambrette using wild-type AB and transgenic Tg(fli1a:EGFP)y1 zebrafish. Different concentrations were delivered to zebrafish by direct soaking from 6 to 72 h post-fertilization (hpf). The LC50 of musk ambrette was 76.4 µg mL-1. As musk ambrette concentration increased, zebrafish embryos showed developmental delays (50 µg mL-1, 22 hpf), pericardial edema (5 µg mL-1, 48 hpf), circulatory disturbances, curved body axis (1 µg mL-1, 72 hpf) and death (100 µg mL-1, 22 hpf). Target organ toxicity was evaluated by a zebrafish angiogenesis model. Musk ambrette induced cardiovascular morphological changes, vessel permeability variation, angiogenic changes and cardiotoxicity (10 µg mL-1, 48 hpf). The disappearance of caudal vein plexus confirmed the vascular development toxicity. Musk ambrette negatively affects early life-stage survival and demonstrates various toxicities in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping