PUBLICATION

Acrylamide acute neurotoxicity in adult zebrafish

Authors
Faria, M., Ziv, T., Gómez-Canela, C., Ben-Lulu, S., Prats, E., Novoa-Luna, K.A., Admon, A., Piña, B., Tauler, R., Gómez-Oliván, L.M., Raldúa, D.
ID
ZDB-PUB-180523-1
Date
2018
Source
Scientific Reports   8: 7918 (Journal)
Registered Authors
Piña, Benjamin, Raldúa, Demetrio
Keywords
none
MeSH Terms
  • Acrylamide/toxicity*
  • Acute Disease
  • Animals
  • Behavior, Animal
  • Gene Expression Regulation/drug effects*
  • Neurotoxicity Syndromes/etiology
  • Neurotoxicity Syndromes/metabolism*
  • Neurotoxicity Syndromes/pathology
  • Proteome/drug effects*
  • Transcriptome/drug effects*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
29784925 Full text @ Sci. Rep.
CTD
29784925
Abstract
Acute exposure to acrylamide (ACR), a type-2 alkene, may lead to a ataxia, skeletal muscles weakness and numbness of the extremities in human and laboratory animals. In the present manuscript, ACR acute neurotoxicity has been characterized in adult zebrafish, a vertebrate model increasingly used in human neuropharmacology and toxicology research. At behavioral level, ACR-treated animals exhibited "depression-like" phenotype comorbid with anxiety behavior. At transcriptional level, ACR induced down-regulation of regeneration-associated genes and up-regulation of oligodendrocytes and reactive astrocytes markers, altering also the expression of genes involved in the presynaptic vesicle cycling. ACR induced also significant changes in zebrafish brain proteome and formed adducts with selected cysteine residues of specific proteins, some of them essential for the presynaptic function. Finally, the metabolomics analysis shows a depletion in the monoamine neurotransmitters, consistent with the comorbid depression and anxiety disorder, in the brain of the exposed fish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping