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ZFIN ID: ZDB-PUB-180518-7
Palmitate induces nitric oxide production and inflammatory cytokine expression in zebrafish
Cha, S.H., Hwang, Y., Kim, K.N., Jun, H.S.
Date: 2018
Source: Fish & shellfish immunology 79: 163-167 (Journal)
Registered Authors:
Keywords: fish immunology, free fatty acid, inflammation, palmitate, zebrafish diabetic model
MeSH Terms:
  • Animals
  • Biomarkers/metabolism
  • Cytokines/genetics*
  • Cytokines/metabolism
  • Diabetes Mellitus, Type 2/chemically induced
  • Diabetes Mellitus, Type 2/immunology*
  • Disease Models, Animal
  • Embryo, Nonmammalian/immunology
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Inflammation/chemically induced
  • Inflammation/immunology*
  • Nitric Oxide/metabolism*
  • Palmitic Acid/administration & dosage
  • Zebrafish/immunology*
PubMed: 29772372 Full text @ Fish Shellfish Immunol.
Inflammation markers in zebrafish embryos reflect a toxic response that is common to other animal models and humans. Free fatty acids (FFAs) are known to cause damage in various tissues by inducing inflammation. In this study, we investigated whether a FFA (palmitate) induces inflammation in zebrafish embryos. Nitrous oxide (NO) production and cyclooxygenase-2 (COX-2) mRNA expression were increased in palmitate-treated zebrafish embryos in a dose-dependent manner. mRNA expression of pro-inflammatory cytokines, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF- α), were also increased. Additionally, the mRNA expression of p65 nuclear factor-kB and I-kB-α were significantly increased after palmitate-treatment. Increased reactive oxygen species (ROS) expression was observed in palmitate-treated zebrafish embryos as well as pericardial edema. Additionally, mRNA expression of pro-inflammatory cytokines were increased in zebrafish liver and pancreas fed with palmitate-contained diet. Taken together, these results indicated that palmitate increases pro-inflammatory mediators in zebrafish embryos, suggesting that zebrafish could be an alternative animal model for inflammatory disease including diabetes.