PUBLICATION
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
- Authors
- Silic, M.R., Zhang, G.
- ID
- ZDB-PUB-180515-6
- Date
- 2018
- Source
- Journal of visualized experiments : JoVE (134): (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Zebrafish/embryology*
- Neoplasms/diagnosis*
- Animals, Genetically Modified
- Electrophysiological Phenomena/physiology*
- DNA Transposable Elements
- Animals
- Gene Transfer Techniques
- PubMed
- 29757272 Full text @ J. Vis. Exp.
Citation
Silic, M.R., Zhang, G. (2018) Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors. Journal of visualized experiments : JoVE. (134).
Abstract
Bioelectricity, endogenous electrical signaling mediated by ion channels and pumps located on the cell membrane, plays important roles in signaling processes of excitable neuronal and muscular cells and many other biological processes, such as embryonic developmental patterning. However, there is a need for in vivo electrical activity monitoring in vertebrate embryogenesis. The advances of genetically encoded fluorescent voltage indicators (GEVIs) have made it possible to provide a solution for this challenge. Here, we describe how to create a transgenic voltage indicator zebrafish using the established voltage indicator, ASAP1 (Accelerated Sensor of Action Potentials 1), as an example. The Tol2 kit and a ubiquitous zebrafish promoter, ubi, were chosen in this study. We also explain the processes of Gateway site-specific cloning, Tol2 transposon-based zebrafish transgenesis, and the imaging process for early-stage fish embryos and fish tumors using regular epifluorescent microscopes. Using this fish line, we found that there are cellular electric voltage changes during zebrafish embryogenesis, and fish larval movement. Furthermore, it was observed that in a few zebrafish malignant peripheral nerve sheath tumors, the tumor cells were generally polarized compared to the surrounding normal tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping