PUBLICATION
Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish.
- Authors
- Garcia Campoy, A.H., Perez Gutierrez, R.M., Manriquez-Alvirde, G., Muñiz Ramirez, A.
- ID
- ZDB-PUB-180512-6
- Date
- 2018
- Source
- International Journal of Nanomedicine 13: 2601-2612 (Journal)
- Registered Authors
- Keywords
- Eysenhardtia polystachya, diabetes, hyperlipidemia, insulin, silver nanoparticles, zebrafish
- MeSH Terms
-
- Animals
- Cell Line
- Diabetes Mellitus, Experimental/drug therapy
- Diabetes Mellitus, Experimental/etiology
- Fabaceae/chemistry*
- Hydrogen Peroxide/toxicity
- Hypoglycemic Agents/administration & dosage
- Hypoglycemic Agents/pharmacology*
- Insulin
- Insulin-Secreting Cells/drug effects
- Insulin-Secreting Cells/pathology
- Metal Nanoparticles/administration & dosage
- Metal Nanoparticles/chemistry*
- Mice
- Microscopy, Electron, Transmission
- Plant Extracts/administration & dosage
- Plant Extracts/chemistry
- Plant Extracts/pharmacology*
- Silver/chemistry
- Spectrophotometry, Ultraviolet
- Zebrafish
- PubMed
- 29750032 Full text @ Int. J. Nanomedicine
Citation
Garcia Campoy, A.H., Perez Gutierrez, R.M., Manriquez-Alvirde, G., Muñiz Ramirez, A. (2018) Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish.. International Journal of Nanomedicine. 13:2601-2612.
Abstract
Background The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus.
Materials and methods EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques.
Results Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased. The synthesized EP/AgNPs showed an absorption peak at 413 nm on ultraviolet-visible spectroscopy, revealing the surface plasmon resonance of the nanoparticles. Transmission electron microscopy indicated that most of the particles were spherical, with a diameter of 10-12 nm, a polydispersity index of 0.197, and a zeta potential of -32.25 mV, suggesting high stability of the nanoparticles. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury.
Conclusion The results indicate that EP/AgNPs have good antidiabetic activity and therefore could be used to prevent the development of diabetes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping