PUBLICATION
Identification and expression of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in zebrafish Danio rerio: Implication for age-related biomarkers
- Authors
- Feng, S., Wang, S., Wang, Y., Yang, Q., Wang, D., Li, H.
- ID
- ZDB-PUB-180509-29
- Date
- 2018
- Source
- Gene expression patterns : GEP 29: 47-58 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- Age-related markers, Carbonic anhydrase, Myosin regulatory light chain, Selenium-binding protein, Zebrafish
- MeSH Terms
-
- Age Factors
- Amino Acid Sequence
- Animals
- Biomarkers/metabolism*
- Carbonic Anhydrases/genetics
- Carbonic Anhydrases/metabolism
- Computational Biology
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Gene Expression Regulation, Developmental*
- Myosin Light Chains/genetics
- Myosin Light Chains/metabolism
- Organ Specificity
- Phylogeny
- Selenium-Binding Proteins/genetics
- Selenium-Binding Proteins/metabolism
- Sequence Homology
- Spatio-Temporal Analysis
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29738878 Full text @ Gene Expr. Patterns
Citation
Feng, S., Wang, S., Wang, Y., Yang, Q., Wang, D., Li, H. (2018) Identification and expression of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in zebrafish Danio rerio: Implication for age-related biomarkers. Gene expression patterns : GEP. 29:47-58.
Abstract
Proteomic study has determined age-related changes in synthesis of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in muscle of post-menopausal women. However, little information is available regarding the expression and role of these proteins in early development and life span. In this study we showed that zebrafish ca2, myl2a, myl2b and selenbp1 were highly identical to their mammalian counterparts in primary and tertiary structures as well as genomic organization and syntenic map. They displayed distinct spatiotemporal expression patterns in embryos and larvae of zebrafish. Moreover, their transcription levels in the respective tissues were obviously remodeled in an age-dependent fashion, i.e. some mRNA levels were increased, while others remained unchanged or even decreased, suggesting that CA2, MYL2a, MYL2b and SELENBP1 can be used as aging biomarkers. Our study also lays a foundation for further illumination of the functions of these genes in early development and aging processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping