PUBLICATION
Cytochrome P450 2AA molecular clone, expression pattern, and different regulation by fish oil and lard oil in diets of grass carp (Ctenopharyngodon idella)
- Authors
- Shi, X.C., Sun, J., Jin, A., Ji, H., Yu, H.B., Li, Y., Li, X.X., Liu, P., Li, C., Huang, J.Q.
- ID
- ZDB-PUB-180508-6
- Date
- 2018
- Source
- Fish physiology and biochemistry 44(4): 1019-1026 (Journal)
- Registered Authors
- Keywords
- CYP2AA, Ctenopharyngodon idellus, Fish oil, Lipid metabolism
- MeSH Terms
-
- Dietary Fats/administration & dosage
- Amino Acid Sequence
- Diet*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Fish Oils/administration & dosage
- Organ Specificity
- Liver/drug effects
- Liver/metabolism
- Sequence Homology
- Animals
- Adipose Tissue/drug effects
- Adipose Tissue/metabolism
- Carps/growth & development
- Carps/metabolism*
- Base Sequence
- Gene Expression Regulation
- Fish Proteins/genetics*
- Transcriptome*
- Cytochrome P-450 Enzyme System/genetics*
- Amino Acid Motifs
- Cloning, Molecular
- PubMed
- 29725939 Full text @ Fish Physiol. Biochem.
Citation
Shi, X.C., Sun, J., Jin, A., Ji, H., Yu, H.B., Li, Y., Li, X.X., Liu, P., Li, C., Huang, J.Q. (2018) Cytochrome P450 2AA molecular clone, expression pattern, and different regulation by fish oil and lard oil in diets of grass carp (Ctenopharyngodon idella). Fish physiology and biochemistry. 44(4):1019-1026.
Abstract
Cytochrome P450 enzymes (CYP enzymes) catalyze important metabolic reactions of exogenous and endogenous substrates, including fatty acid. In this study, we cloned the complete CDS of the cytochrome P450 2AA (CYP2AA) gene from the grass carp (Ctenopharyngodon idella) for the first time. CYP2AA consisted of 1500 bp, which encoded a predicted protein of 499 amino acids. The identities of CYP2AA between C. idella and zebrafish were 86%. It consists of the conserved heme-binding motif FXXGXXXCXG. Quantitative real-time PCR analysis indicated that CYP2AA mRNA in C. idella was highly expressed in liver and adipose tissue. The effects of fish oil and lard oil in diets on expression of CYP2AA mRNA in vivo were also investigated. The fish oil (FO) group exhibited significantly higher CYP2AA expression in adipose tissue than the lard oil (LO) group (P?
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping