PUBLICATION
Fenbuconazole exposure impacts the development of zebrafish embryos
- Authors
- Wu, Y., Yang, Q., Chen, M., Zhang, Y., Zuo, Z., Wang, C.
- ID
- ZDB-PUB-180502-21
- Date
- 2018
- Source
- Ecotoxicology and environmental safety 158: 293-299 (Journal)
- Registered Authors
- Keywords
- Embryonic development, Fish, Mechanism, Triazole fungicide
- MeSH Terms
-
- Dose-Response Relationship, Drug
- Male
- Triazoles/toxicity*
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Muscle, Skeletal/drug effects
- Muscle, Skeletal/embryology
- Heart/drug effects
- Heart/embryology
- Larva/drug effects
- Animals
- Up-Regulation
- Troponin T/genetics
- Troponin T/metabolism
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Homeobox Protein Nkx-2.5/genetics
- Homeobox Protein Nkx-2.5/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Female
- Nitriles/toxicity*
- GATA Transcription Factors/genetics
- GATA Transcription Factors/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- Bone Morphogenetic Protein 2/genetics
- Bone Morphogenetic Protein 2/metabolism
- PubMed
- 29715634 Full text @ Ecotoxicol. Environ. Saf.
Citation
Wu, Y., Yang, Q., Chen, M., Zhang, Y., Zuo, Z., Wang, C. (2018) Fenbuconazole exposure impacts the development of zebrafish embryos. Ecotoxicology and environmental safety. 158:293-299.
Abstract
Fenbuconazole (FBZ), a triazole-containing fungicide, is widely used in agriculture and horticulture. In the present study, the development and cardiac functioning were observed and determined in zebrafish embryos exposed to FBZ at 5, 50 and 500?ng/L nominal concentrations for 72?h. The results showed that 500?ng/L FBZ significantly increased pericardial edema rate, spine curvature rate, disturbed cardiac function, and led a shortened lower jaw. The transcription of genes such as tbx5, nkx2.5, tnnt2, gata4, bmp2b, myl7 was altered, which might be responsible for the cardiac developmental and functioning defects in the larvae. The deformation in bone development might be related with the impaired transcription levels of shh and bmp2b. The transcription of cyp26a1 (encoding retinoic acid metabolism enzyme) was significantly up-regulated in the 500?ng/L group, which might be a reason causing the teratogenic effect of FBZ. These results suggest that FBZ could have toxic effects on embryonic development, which should be considered in the risk evaluation of FBZ application.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping