PUBLICATION
Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate
- Authors
- Wu, C., Gao, J., Chen, L., Shao, X., Ye, J.
- ID
- ZDB-PUB-180426-2
- Date
- 2018
- Source
- Fish physiology and biochemistry 44(4): 1127-1141 (Journal)
- Registered Authors
- Keywords
- Adiponectin receptor, Dietary carbohydrate, Mylopharyngodon piceus, cDNA cloning, mRNA expression
- MeSH Terms
-
- Dietary Carbohydrates/administration & dosage*
- Receptors, Adiponectin/genetics
- Receptors, Adiponectin/metabolism*
- Cloning, Molecular
- Organ Specificity
- Animals
- Phylogeny
- Gene Expression Regulation*
- Sequence Homology
- Carps/genetics
- Carps/growth & development
- Carps/metabolism*
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Amino Acid Sequence
- PubMed
- 29687170 Full text @ Fish Physiol. Biochem.
Citation
Wu, C., Gao, J., Chen, L., Shao, X., Ye, J. (2018) Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate. Fish physiology and biochemistry. 44(4):1127-1141.
Abstract
In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, respectively. BLAST analysis reveals that MpAdipoRs share high identities with other known AdipoRs from zebrafish, rainbow trout, human, etc. And there were all seven transmembrane regions in the amino acid sequences of MpAdipoR1s and MpAdipoR2, respectively. The relative expression levels of MpAdipoR1s were higher in the liver, blood, brain, and eyes in black carp (p ?0.05). However, the expression levels of MpAdipoRs in the adipose tissues were significantly downregulated at excessive dietary CHO (37.91%) compared with other groups (p
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping