PUBLICATION
Retinoic Acid Maintains Function of Neural Crest-Derived Ocular and Craniofacial Structures in Adult Zebrafish
- Authors
- Chawla, B., Swain, W., Williams, A.L., Bohnsack, B.L.
- ID
- ZDB-PUB-180421-17
- Date
- 2018
- Source
- Investigative ophthalmology & visual science 59: 1924-1935 (Journal)
- Registered Authors
- Bohnsack, Brenda, Williams, Antionette
- Keywords
- none
- MeSH Terms
-
- Animals
- Anterior Eye Segment/drug effects
- Anterior Eye Segment/pathology
- Antineoplastic Agents/pharmacology*
- Apoptosis
- Aqueous Humor/physiology
- Craniofacial Abnormalities/prevention & control*
- Female
- Gene Expression Regulation, Developmental/physiology
- In Situ Nick-End Labeling
- Male
- Neural Crest/drug effects*
- Nystagmus, Optokinetic/drug effects
- Nystagmus, Optokinetic/physiology
- Real-Time Polymerase Chain Reaction
- Tretinoin/pharmacology*
- Vision, Ocular/drug effects*
- Vision, Ocular/physiology
- Zebrafish
- PubMed
- 29677354 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
Chawla, B., Swain, W., Williams, A.L., Bohnsack, B.L. (2018) Retinoic Acid Maintains Function of Neural Crest-Derived Ocular and Craniofacial Structures in Adult Zebrafish. Investigative ophthalmology & visual science. 59:1924-1935.
Abstract
Purpose Retinoic acid (RA) is required for embryonic formation of the anterior segment of the eye and craniofacial structures. The present study further investigated the role of RA in maintaining the function of these neural crest-derived structures in adult zebrafish.
Methods Morphology and histology were analyzed by using live imaging, methylacrylate sections, and TUNEL assay. Functional analysis of vision and aqueous humor outflow were assayed with real-time imaging.
Results Both decreased and increased RA signaling altered craniofacial and ocular structures in adult zebrafish. Exogenous treatment with all-trans RA for 5 days resulted in a prognathic jaw, while inhibition of endogenous RA synthesis through treatment with 4-diethylaminobenzaldehyde (DEAB) decreased head height. In adult eyes, RA activity was localized to the retinal pigment epithelium, photoreceptors, outer plexiform layer, inner plexiform layer, iris stroma, and ventral canalicular network. Exogenous RA increased apoptosis in the iris stroma and canalicular network in the ventral iridocorneal angle, resulting in the loss of these structures and decreased aqueous outflow. DEAB, which decreased RA activity throughout the eye, induced widespread apoptosis, resulting in corneal edema, cataracts, retinal atrophy, and loss of iridocorneal angle structures. DEAB-treated fish were blind with no optokinetic response and no aqueous outflow from the anterior chamber.
Conclusions Tight control of RA levels is required for normal structure and function of the adult anterior segment. These studies demonstrated that RA plays an important role in maintaining ocular and craniofacial structures in adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping