ZFIN ID: ZDB-PUB-180421-12
Mutant MYO1F alters the mitochondrial network and induces tumor proliferation in thyroid cancer
Diquigiovanni, C., Bergamini, C., Evangelisti, C., Isidori, F., Vettori, A., Tiso, N., Argenton, F., Costanzini, A., Iommarini, L., Anbunathan, H., Pagotto, U., Repaci, A., Babbi, G., Casadio, R., Lenaz, G., Rhoden, K.J., Porcelli, A.M., Fato, R., Bowcock, A., Seri, M., Romeo, G., Bonora, E.
Date: 2018
Source: International Journal of Cancer   143(7): 1706-1719 (Journal)
Registered Authors: Argenton, Francesco, Tiso, Natascia, Vettori, Andrea
Keywords: MYO1F, Non-Medullary Thyroid Carcinoma, TCO locus, mitochondrial network, whole exome sequencing
MeSH Terms:
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Apoptosis
  • Cell Proliferation*
  • Cells, Cultured
  • Child
  • Chromosomes, Human, Pair 19
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology*
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Mitochondria/genetics
  • Mitochondria/metabolism
  • Mitochondria/pathology*
  • Mutation*
  • Myosin Type I/chemistry
  • Myosin Type I/genetics*
  • Myosin Type I/metabolism
  • Oxygen Consumption
  • Pedigree
  • Protein Conformation
  • Thyroid Cancer, Papillary/genetics
  • Thyroid Cancer, Papillary/metabolism
  • Thyroid Cancer, Papillary/pathology*
  • Thyroid Neoplasms/genetics
  • Thyroid Neoplasms/metabolism
  • Thyroid Neoplasms/pathology*
  • Young Adult
  • Zebrafish
PubMed: 29672841 Full text @ Int. J. Cancer
ABSTRACT
Familial aggregation is a significant risk factor for the development of thyroid cancer and Familial Non-Medullary Thyroid Cancer (FNMTC) accounts for 5-7% of all NMTC. Whole Exome Sequencing analysis in the family affected by FNMTC with oncocytic features where our group previously identified a predisposing locus on chromosome 19p13.2, revealed a novel heterozygous mutation (c.400G>A, NM_012335; p.Gly134Ser) in exon 5 of MYO1F, mapping to the linkage locus. In the thyroid FRTL-5 cell model stably expressing the mutant MYO1F p.Gly134Ser protein we observed an altered mitochondrial network, with increased mitochondrial mass and a significant increase of both intracellular and extracellular Reactive Oxygen Species, compared to cells expressing the wild-type protein or carrying the empty vector. The mutation conferred a significant advantage in colony formation, invasion and anchorage independent growth. These data were corroborated by in vivo studies in zebrafish, since we demonstrated that the mutant MYO1F p.Gly134Ser, when overexpressed, can induce proliferation in whole vertebrate embryos, compared to the wild-type one. MYO1F screening in additional 192 FNMTC families identified another variant in exon 7, which leads to exon skipping, and is predicted to alter the ATP-binding domain in MYO1F. Our study identified for the first time a role for MYO1F in NMTC. This article is protected by copyright. All rights reserved.
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