PUBLICATION
An Integrative Approach Combining Passive Sampling, Bioassays and Effect-Directed Analysis to Assess the Impact of Wastewater Effluent
- Authors
- Sonavane, M., Schollée, J.E., Hidasi, A.O., Creusot, N., Brion, F., Suter, M.J., Hollender, J., Aït-Aïssa, S.
- ID
- ZDB-PUB-180419-4
- Date
- 2018
- Source
- Environmental toxicology and chemistry 37(8): 2079-2088 (Journal)
- Registered Authors
- Keywords
- Effect-directed analysis (EDA), Embryo toxicity, Endocrine disrupting chemicals (EDCs), LC-MS/MS analysis, Passive sampling, WWTP effluent
- MeSH Terms
-
- Animals
- Biological Assay/methods*
- Endocrine Disruptors/analysis
- Endocrine Disruptors/toxicity
- Environmental Monitoring/methods*
- Estrogens/analysis
- Rivers/chemistry
- Toxicity Tests
- Wastewater/chemistry*
- Water Pollutants, Chemical/analysis*
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology
- PubMed
- 29667746 Full text @ Environ. Toxicol. Chem.
Citation
Sonavane, M., Schollée, J.E., Hidasi, A.O., Creusot, N., Brion, F., Suter, M.J., Hollender, J., Aït-Aïssa, S. (2018) An Integrative Approach Combining Passive Sampling, Bioassays and Effect-Directed Analysis to Assess the Impact of Wastewater Effluent. Environmental toxicology and chemistry. 37(8):2079-2088.
Abstract
Wastewater treatment plant (WWTP) effluents are major sources of endocrine disrupting chemicals (EDCs) and of other chemicals of toxicological concern for the aquatic environment. In this study, we used an integrated strategy combining passive sampling (Chemcatcher®), developmental toxicity and mechanism-based in vitro and in vivo bioassays to monitor the impacts of a WWTP on a river. In vitro screening revealed the WWTP effluent as a source of estrogen (ER), glucocorticoid (GR), aryl hydrocarbon (AhR) receptor mediated activities, impacting the downstream river site where significant activities were also measured, albeit to a lesser extent than in the effluent. Effect-directed analysis (EDA) of the effluent successfully identified the presence of potent estrogens (estrone, 17α-ethinylestradiol and 17β-estradiol) and glucocorticoids (clobetasol propionate and fluticasone propionate) as the major contributors to the observed in vitro activities, even though other unidentified active chemicals were likely present. The impact of the WWTP was also assessed using zebrafish embryo assays, highlighting its ability to induce estrogenic response through up-regulation of aromatase promoter-dependent reporter gene in the transgenic (cyp19a1b-GFP) zebrafish assay and to generate teratogenic effects at non-lethal concentrations in the zebrafish embryo toxicity test. This study argues for the use of such an integrated approach, combining passive sampling, bioassays and EDA, to comprehensively identify endocrine active compounds and associated hazards of WTTP effluents. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping