PUBLICATION
Cardiac voltage-gated sodium channel expression and electrophysiological characterization of the sodium current in the zebrafish (Danio rerio) ventricle
- Authors
- Haverinen, J., Hassinen, M., Korajoki, H., Vornanen, M.
- ID
- ZDB-PUB-180418-46
- Date
- 2018
- Source
- Progress in Biophysics and Molecular Biology 138: 59-68 (Journal)
- Registered Authors
- Keywords
- Cardiac myocytes, Electrical excitability, Gene expression, MS-222 sensitivity, Patch-clamp, Tetrodotoxin sensitivity
- MeSH Terms
-
- Aminobenzoates/pharmacology
- Animals
- Electrophysiological Phenomena*/drug effects
- Gene Expression Regulation*
- Heart Ventricles/drug effects
- Heart Ventricles/metabolism*
- Kinetics
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sodium/metabolism*
- Tetrodotoxin/pharmacology
- Ventricular Function*/drug effects
- Voltage-Gated Sodium Channels/metabolism*
- Zebrafish*
- PubMed
- 29655910 Full text @ Prog. Biophys. Mol. Biol.
Citation
Haverinen, J., Hassinen, M., Korajoki, H., Vornanen, M. (2018) Cardiac voltage-gated sodium channel expression and electrophysiological characterization of the sodium current in the zebrafish (Danio rerio) ventricle. Progress in Biophysics and Molecular Biology. 138:59-68.
Abstract
Na+ channel α-subunit composition of the zebrafish heart and electrophysiological properties of Na+ current (INa) of zebrafish ventricular myocytes were examined. Eight Na+ channel α-subunits were expressed in both atrium and ventricle of the zebrafish heart. Nav1.5Lb, an orthologue to the human Nav1.5, was clearly the predominant isoform in both chambers representing 65.2 ± 4.1% and 83.1 ± 2.1% of all Na+ channel transcripts in atrium and ventricle, respectively. Nav1.4b, an orthologue to human Nav1.4, formed 34.1 ± 4.1 and 16.2 ± 2.0% of the Na+ channel transcripts in atrium and ventricle, respectively. The density of INa and the rate of action potential upstroke in zebrafish ventricular myocytes at 28 °C were similar to those of human ventricles at the comparable temperature. Na+ channel isoforms and the main electrophysiological characteristics of the INa are largely similar in zebrafish and human hearts indicating evolutionary conservation of Na+ channel composition and function. The zebrafish INa differs from the human cardiac INa in terms of higher tetrodotoxin sensitivity (IC50-value = 5.3 ± 0.1 nM) and slower inactivation kinetics. The zebrafish INa was inhibited with tricaine (MS-222) with an IC50-value of 1.2 ± 0.18 mM (336 mg l-1), suggesting some care in the use of MS-222 as an anesthetic.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping