PUBLICATION
Safety assessment of Bacillus thuringiensis (Bt) insecticidal proteins Cry1C and Cry2A with a zebrafish embryotoxicity test
- Authors
- Gao, Y.J., Zhu, H.J., Chen, Y., Li, Y.H., Peng, Y.F., Chen, X.P.
- ID
- ZDB-PUB-180418-38
- Date
- 2018
- Source
- Journal of Agricultural and Food Chemistry 66(17): 4336-4344 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/genetics
- Bacillus thuringiensis/chemistry
- Bacterial Proteins/genetics
- Bacterial Proteins/toxicity*
- Crops, Agricultural
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/drug effects
- Endotoxins/genetics
- Endotoxins/toxicity*
- Hemolysin Proteins/genetics
- Hemolysin Proteins/toxicity*
- Insecticides/toxicity*
- Oxidative Stress/genetics
- Plants, Genetically Modified/adverse effects*
- Plants, Genetically Modified/chemistry
- Plants, Genetically Modified/genetics
- RNA, Messenger/analysis
- Water Pollution
- Zebrafish/embryology*
- PubMed
- 29653490 Full text @ J. Agric. Food Chem.
- CTD
- 29653490
Citation
Gao, Y.J., Zhu, H.J., Chen, Y., Li, Y.H., Peng, Y.F., Chen, X.P. (2018) Safety assessment of Bacillus thuringiensis (Bt) insecticidal proteins Cry1C and Cry2A with a zebrafish embryotoxicity test. Journal of Agricultural and Food Chemistry. 66(17):4336-4344.
Abstract
Because of the large-scale planting of transgenic Bt crops, fish would be exposed to freely soluble Bt insecticidal protein(s) that are released from Bt crop tissues into adjacent bodies of water or by way of direct feeding on deposited plant material. To assess the safety of two Bt proteins Cry1C and Cry2A to fish, we used zebrafish as a representative species and exposed their embryos to 0.1, 1, and 10 mg/L of the two Cry proteins until 132 hours post-fertilization, and then several developmental, biochemical, and molecular parameters were evaluated. Chlorpyrifos (CPF), a known toxicant to aquatic organisms, was used as a positive control. Although CPF exposure resulted in significant developmental, biochemical, and molecular changes in the zebrafish embryos, there were almost no significant differences after Cry1C or Cry2A exposure. Thus, we conclude that zebrafish embryos are not sensitive to Cry1C and Cry2A insecticidal proteins at test concentration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping