PUBLICATION
Synaptically silent sensory hair cells in zebrafish are recruited after damage
- Authors
- Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S.
- ID
- ZDB-PUB-180418-24
- Date
- 2018
- Source
- Nature communications 9: 1388 (Journal)
- Registered Authors
- Beirl, Alisha, Kindt, Katie, Li, Suna, Wong, Hiu-Tung Candy, Zhang, Qiuxiang
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Channels, L-Type/genetics
- Calcium Channels, L-Type/metabolism*
- Cations, Divalent
- Embryo, Nonmammalian
- Hair Cells, Auditory/cytology*
- Hair Cells, Auditory/metabolism
- Ion Transport
- Larva/cytology
- Larva/metabolism
- Lateral Line System/growth & development
- Lateral Line System/injuries
- Lateral Line System/metabolism
- Mechanotransduction, Cellular/physiology*
- Potassium/metabolism
- Regeneration/physiology
- Synapses/metabolism*
- Synaptic Transmission/physiology*
- Zebrafish
- PubMed
- 29643351 Full text @ Nat. Commun.
Citation
Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S. (2018) Synaptically silent sensory hair cells in zebrafish are recruited after damage. Nature communications. 9:1388.
Abstract
Analysis of mechanotransduction among ensembles of sensory hair cells in vivo is challenging in many species. To overcome this challenge, we used optical indicators to investigate mechanotransduction among collections of hair cells in intact zebrafish. Our imaging reveals a previously undiscovered disconnect between hair-cell mechanosensation and synaptic transmission. We show that saturating mechanical stimuli able to open mechanically gated channels are unexpectedly insufficient to evoke vesicle fusion in the majority of hair cells. Although synaptically silent, latent hair cells can be rapidly recruited after damage, demonstrating that they are synaptically competent. Therefore synaptically silent hair cells may be an important reserve that acts to maintain sensory function. Our results demonstrate a previously unidentified level of complexity in sculpting sensory transmission from the periphery.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping