PUBLICATION
Toxicological assessment of PEGylated single-walled carbon nanotubes in early developing zebrafish
- Authors
- Cordeiro, M.F., Girardi, F.A., Gonçalves, C.O.F., Peixoto, C.S., Bosco, L.D., Sahoo, S.K., Santos, A.P., Fantini, C., Bruch, G.E., Horn, A.P., Barros, D.M.
- ID
- ZDB-PUB-180403-5
- Date
- 2018
- Source
- Toxicology and applied pharmacology 347: 54-59 (Journal)
- Registered Authors
- Barros, Daniela Marti
- Keywords
- Carbon nanomaterials, Danio rerio, Embriotoxicity, Nanotoxicology, Polyethylene glycol
- MeSH Terms
-
- Animals
- DNA Damage
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/pathology
- Embryonic Development/drug effects
- Larva/drug effects*
- Larva/metabolism
- Molecular Weight
- Nanotubes, Carbon/toxicity*
- Oxidative Stress/drug effects
- Polyethylene Glycols/toxicity*
- Reactive Oxygen Species/metabolism
- Time Factors
- Toxicology/methods*
- Zebrafish/embryology*
- PubMed
- 29609001 Full text @ Tox. App. Pharmacol.
Citation
Cordeiro, M.F., Girardi, F.A., Gonçalves, C.O.F., Peixoto, C.S., Bosco, L.D., Sahoo, S.K., Santos, A.P., Fantini, C., Bruch, G.E., Horn, A.P., Barros, D.M. (2018) Toxicological assessment of PEGylated single-walled carbon nanotubes in early developing zebrafish. Toxicology and applied pharmacology. 347:54-59.
Abstract
Functionalization of single-walled carbon nanotubes (SWCNT) with polyethylene glycol (PEG) is among the most promising strategies to avoid SWCNT aggregation in aqueous media, improving its interactions with biological systems. However, the best molecular PEG weight and functionalization strategy remain under investigation. In this work we assessed the toxicological effects of SWCNT functionalized with PEG at 600 Da in zebrafish embryos. Embryos were exposed to SWCNT at 0.01, 0.1 and 1 mg/L from 3 to 96 h post-fertilization (hpf). At the highest concentration, SWCNT led to toxic effects at several endpoints, including mortality, delayed hatching, malformations, reduced body length, increased ROS production and DNA damage. Even with these effects, SWCNT could not be detected within the bodily tissues of the larvae. Our results give evidence that the tested PEGylation approach was unsuitable to avoid SWCNT aggregation in aqueous media, and that SWCNT can induce toxicity even without being absorbed by the organism by obstructing the chorion pores.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping