PUBLICATION
Effects of short term lead exposure on gut microbiota and hepatic metabolism in adult zebrafish
- Authors
- Xia, J., Lu, L., Jin, C., Wang, S., Zhou, J., Ni, Y., Fu, Z., Jin, Y.
- ID
- ZDB-PUB-180327-1
- Date
- 2018
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 209: 1-8 (Journal)
- Registered Authors
- Keywords
- Gut microbiota, Hepatic metabolism, Lead, Zebrafish
- MeSH Terms
-
- Gene Expression Regulation/drug effects*
- Lead Poisoning/metabolism
- Lead Poisoning/microbiology
- Lead Poisoning/pathology
- Lead Poisoning/physiopathology*
- Firmicutes/classification
- Firmicutes/drug effects
- Firmicutes/growth & development
- Organometallic Compounds/toxicity
- Water Pollutants, Chemical/toxicity
- Gastrointestinal Microbiome/drug effects*
- Molecular Typing
- Toxicity Tests, Acute
- Osmolar Concentration
- Dysbiosis/etiology*
- Intestinal Mucosa/drug effects*
- Intestinal Mucosa/microbiology
- Intestinal Mucosa/pathology
- Alphaproteobacteria/classification
- Alphaproteobacteria/drug effects
- Alphaproteobacteria/growth & development
- Lipid Metabolism
- Energy Metabolism/drug effects*
- Liver/drug effects*
- Liver/metabolism
- Metabolomics/methods
- Zebrafish
- Male
- Mucus
- Glycolysis/drug effects
- Animals
- Fish Proteins/antagonists & inhibitors
- Fish Proteins/genetics
- Fish Proteins/metabolism
- PubMed
- 29574035 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Xia, J., Lu, L., Jin, C., Wang, S., Zhou, J., Ni, Y., Fu, Z., Jin, Y. (2018) Effects of short term lead exposure on gut microbiota and hepatic metabolism in adult zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 209:1-8.
Abstract
Lead (Pb) is one of the most prevalent toxic, nonessential heavy metals that has been associated with a wide range of toxic effects in humans and environmental animals. Here, effects of short time exposure to 10 and 30??g/L Pb on gut microbiota and hepatic metabolism were analyzed in adult male zebrafish. We observed that both 10 and 30??g/L Pb increased the volume of mucus in the gut. At phylum level, the abundance of ?-Proteobacteria decreased significantly and the abundance of Firmicutes increased significantly in the gut when treated with 30??g/L Pb for 7?days. In addition, the 16S rRNA gene sequencing for V3-V4 region revealed a significant change in the richness and diversity of gut microbiota in 30??g/L Pb exposed group. A more depth analysis, at the genus level, discovered that 52 gut microbes identified by operational taxonomic unit analysis were changed significantly in 30??g/L Pb treated group. Based on GC/MS metabolomics analysis, a total of 41 metabolites were significantly altered in 30??g/L Pb treatment group. These changed metabolites were mainly associated with the pathways of glucose and lipid metabolism, amino acid metabolism, nucleotide metabolism. In addition, we also confirmed that the transcription of some genes related to glycolysis and lipid metabolism, including Gk, Aco, Acc1, Fas, Apo and Dgat, decreased significantly in the liver of zebrafish when exposed to 30??g/L Pb for 7?days. Our results observed that Pb could cause gut microbiota dysbiosis and hepatic metabolic disorder in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping