PUBLICATION
Analysis of hpf1 expression and function in early embryonic development of zebrafish
- Authors
- Zhang, Z., Sun, H., Chen, Y., Cao, T., Songyang, Z., Huang, J., Huang, Y.
- ID
- ZDB-PUB-180321-3
- Date
- 2018
- Source
- Development genes and evolution 228(2): 141-147 (Journal)
- Registered Authors
- Keywords
- C4orf27/Hpf1, In situ hybridization, Morpholino, RT-PCR, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Embryonic Development
- Gene Expression Regulation, Developmental*
- Humans
- Models, Animal*
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Poly (ADP-Ribose) Polymerase-1/genetics*
- Sequence Alignment
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 29549427 Full text @ Dev. Genes Evol.
Citation
Zhang, Z., Sun, H., Chen, Y., Cao, T., Songyang, Z., Huang, J., Huang, Y. (2018) Analysis of hpf1 expression and function in early embryonic development of zebrafish. Development genes and evolution. 228(2):141-147.
Abstract
About 70% of zebrafish (Danio rerio) genes are orthologues of the human's, which are of great interests, but still largely unknown for their functions. Recently, a report on human histone PARylation factor 1 (HPF1/C4orf27) showed that it is involved in DNA damage response along with poly (ADP-ribose) polymerase 1 (PARP1). However, its function in living organism remains unclear. Given that zebrafish has showed its values in modeling human diseases and physiology, we characterized a zebrafish homolog of human HPF1 by sequence alignment. We also analyzed its expression pattern during early development and among adult tissues. Furthermore, knocking down hpf1 by morpholinos affected zebrafish early development. Our work provides a novel clue for the mechanism of genome stability and early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping