PUBLICATION
Identification of two Stat3 variants lacking a transactivation domain in grass carp: New insights into alternative splicing in the modification of teleost Stat3 signaling
- Authors
- Wang, X., Du, L., Wei, H., Zhang, A., Yang, K., Zhou, H.
- ID
- ZDB-PUB-180321-18
- Date
- 2018
- Source
- Fish & shellfish immunology 77: 13-21 (Journal)
- Registered Authors
- Keywords
- Grass carp, STAT3 signaling, Stat3?1/2, Teleost-specific splicing, Transcriptional activity
- MeSH Terms
-
- Amino Acid Sequence
- STAT3 Transcription Factor/chemistry
- STAT3 Transcription Factor/genetics*
- STAT3 Transcription Factor/immunology*
- Immunity, Innate/genetics*
- Signal Transduction
- Sequence Alignment/veterinary
- Animals
- Alternative Splicing
- Gene Expression Profiling
- Transcriptional Activation
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Carps/genetics*
- Carps/immunology*
- Fish Diseases/immunology*
- Sequence Analysis, DNA/veterinary
- PubMed
- 29555584 Full text @ Fish Shellfish Immunol.
Citation
Wang, X., Du, L., Wei, H., Zhang, A., Yang, K., Zhou, H. (2018) Identification of two Stat3 variants lacking a transactivation domain in grass carp: New insights into alternative splicing in the modification of teleost Stat3 signaling. Fish & shellfish immunology. 77:13-21.
Abstract
Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT family in response to cytokines and growth factors. In mammals, alternative splicing of STAT3 generates STAT3? and STAT3?, which have distinct and overlapping functions. In the previous study, we have identified two spliceforms of Stat3? (Stat3?1 and Stat3?2) possessing all functional domains of Stat3 in grass carp (Ctenopharyngodon idella). In the present study, two Stat3? variants (Stat3?1 and Stat3?2) without C-terminal transactivation domain were isolated from this species, and their transcripts were ubiquitously expressed in all examined tissues with the highest levels in liver. Further studies showed that Stat3?1/2 had the ability to translocate into the nucleus upon activation, indicating their roles in transcriptional regulation. In support of this notion, grass carp Stat3?1 and Stat3?2 displayed the abilities to inhibit Interleukin-10 (Il-10) signaling and competitively impaired the transcriptional activities of Stat3?1/2. In particular, similar to their mammalian counterparts, grass carp Stat3?1 and Stat3?2 could enhance Stat3?1/2 phosphorylation upon cytokine stimulation. Interestingly, stat3?1 and stat3?2 transcripts were also found in zebrafish (Danio rerio) and goldfish (Carassius auratus), and each variant in these teleosts is generated through similar alternative splicing events, including exon skipping and intron retention. This highlights a conserved splicing event of stat3 gene during vertebrate evolution and indicates a potential physiological significance of generating unique Stat3 variants in fish. These results, along with the findings regarding Stat3?1/2, demonstrate the existence of Stat3 isoforms with functional diversity and redundancy in teleosts. It leads to the hypothesis that teleost-specific spliceforms of Stat3 gene may contribute to the complexity of Stat3 signaling in fishes, thereby benefiting them to adapt to evolution and environmental changes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping