PUBLICATION
Identification of two Stat3 variants lacking a transactivation domain in grass carp: New insights into alternative splicing in the modification of teleost Stat3 signaling
- Authors
- Wang, X., Du, L., Wei, H., Zhang, A., Yang, K., Zhou, H.
- ID
- ZDB-PUB-180321-18
- Date
- 2018
- Source
- Fish & shellfish immunology 77: 13-21 (Journal)
- Registered Authors
- Keywords
- Grass carp, STAT3 signaling, Stat3β1/2, Teleost-specific splicing, Transcriptional activity
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Carps/genetics*
- Carps/immunology*
- Fish Diseases/immunology*
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Gene Expression Profiling
- Immunity, Innate/genetics*
- STAT3 Transcription Factor/chemistry
- STAT3 Transcription Factor/genetics*
- STAT3 Transcription Factor/immunology*
- Sequence Alignment/veterinary
- Sequence Analysis, DNA/veterinary
- Signal Transduction
- Transcriptional Activation
- PubMed
- 29555584 Full text @ Fish Shellfish Immunol.
Citation
Wang, X., Du, L., Wei, H., Zhang, A., Yang, K., Zhou, H. (2018) Identification of two Stat3 variants lacking a transactivation domain in grass carp: New insights into alternative splicing in the modification of teleost Stat3 signaling. Fish & shellfish immunology. 77:13-21.
Abstract
Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT family in response to cytokines and growth factors. In mammals, alternative splicing of STAT3 generates STAT3α and STAT3β, which have distinct and overlapping functions. In the previous study, we have identified two spliceforms of Stat3α (Stat3α1 and Stat3α2) possessing all functional domains of Stat3 in grass carp (Ctenopharyngodon idella). In the present study, two Stat3β variants (Stat3β1 and Stat3β2) without C-terminal transactivation domain were isolated from this species, and their transcripts were ubiquitously expressed in all examined tissues with the highest levels in liver. Further studies showed that Stat3β1/2 had the ability to translocate into the nucleus upon activation, indicating their roles in transcriptional regulation. In support of this notion, grass carp Stat3β1 and Stat3β2 displayed the abilities to inhibit Interleukin-10 (Il-10) signaling and competitively impaired the transcriptional activities of Stat3α1/2. In particular, similar to their mammalian counterparts, grass carp Stat3β1 and Stat3β2 could enhance Stat3α1/2 phosphorylation upon cytokine stimulation. Interestingly, stat3β1 and stat3β2 transcripts were also found in zebrafish (Danio rerio) and goldfish (Carassius auratus), and each variant in these teleosts is generated through similar alternative splicing events, including exon skipping and intron retention. This highlights a conserved splicing event of stat3 gene during vertebrate evolution and indicates a potential physiological significance of generating unique Stat3 variants in fish. These results, along with the findings regarding Stat3α1/2, demonstrate the existence of Stat3 isoforms with functional diversity and redundancy in teleosts. It leads to the hypothesis that teleost-specific spliceforms of Stat3 gene may contribute to the complexity of Stat3 signaling in fishes, thereby benefiting them to adapt to evolution and environmental changes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping