PUBLICATION
Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules
- Authors
- Martin, M., Veloso, A., Wu, J., Katrukha, E.A., Akhmanova, A.
- ID
- ZDB-PUB-180317-13
- Date
- 2018
- Source
- eLIFE 7: (Journal)
- Registered Authors
- Keywords
- cell biology, human, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/physiology
- Cell Polarity/physiology*
- Cells, Cultured
- Centrosome/metabolism
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism
- Endothelial Cells/cytology
- Endothelial Cells/metabolism
- Endothelial Cells/physiology*
- Golgi Apparatus/metabolism
- Humans
- Microtubule-Associated Proteins/genetics
- Microtubule-Associated Proteins/metabolism
- Microtubules/metabolism*
- Neovascularization, Physiologic/physiology*
- RNA Interference
- Zebrafish
- PubMed
- 29547120 Full text @ Elife
Citation
Martin, M., Veloso, A., Wu, J., Katrukha, E.A., Akhmanova, A. (2018) Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules. eLIFE. 7.
Abstract
Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, a pivotal role in setting up asymmetry is attributed to the relative positioning of the centrosome and the nucleus. Here, we show that centrosome loss had no effect on the ability of endothelial cells to polarize and move in 2D and 3D environments. In contrast, non-centrosomal microtubules stabilized by the microtubule minus-end-binding protein CAMSAP2 were required for directional migration on 2D substrates and for the establishment of polarized cell morphology in soft 3D matrices. CAMSAP2 was also important for persistent endothelial cell sprouting duringin vivozebrafish vessel development. In the absence of CAMSAP2, cell polarization in 3D could be partly rescued by centrosome depletion, indicating that in these conditions the centrosome inhibited cell polarity. We propose that CAMSAP2-protected non-centrosomal microtubules are needed for establishing cell asymmetry by enabling microtubule enrichment in a single cell protrusion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping