ZFIN ID: ZDB-PUB-180317-10
Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve
Cunningham, R.L., Monk, K.R.
Date: 2018
Source: Methods in molecular biology (Clifton, N.J.) 1739: 385-400 (Chapter)
Registered Authors: Monk, Kelly
Keywords: Adult zebrafish lateral line nerve, Lateral line nerve, Motor nerves, Myelin ultrastructure, Transmission electron microscopy
MeSH Terms:
  • Animals
  • Larva/cytology
  • Larva/ultrastructure
  • Microscopy, Electron, Transmission/methods*
  • Myelin Sheath/metabolism
  • Schwann Cells/cytology
  • Schwann Cells/ultrastructure
  • Zebrafish
PubMed: 29546722 Full text @ Meth. Mol. Biol.
ABSTRACT
Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.
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