Cunningham, R.L., Monk, K.R. (2018) Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve. Methods in molecular biology (Clifton, N.J.). 1739:385-400.
Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.