PUBLICATION
Short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish (Danio rerio)
- Authors
- Cao, F., Wu, P., Huang, L., Li, H., Qian, L., Pang, S., Qiu, L.
- ID
- ZDB-PUB-180313-9
- Date
- 2018
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 198: 129-140 (Journal)
- Registered Authors
- Keywords
- Azoxystrobin, Developmental effects, Potential mechanisms, Zebrafish
- MeSH Terms
-
- Adenosine Triphosphate/metabolism
- Animals
- Antioxidants/metabolism
- Apoptosis/drug effects
- Electron Transport Complex III/metabolism
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation/drug effects
- Immunity, Innate/drug effects
- Larva/anatomy & histology
- Larva/drug effects
- Larva/growth & development
- Liver/drug effects
- Liver/pathology
- Malondialdehyde/metabolism
- Mitochondria/drug effects
- Mitochondria/metabolism
- Mitochondria/ultrastructure
- Oxidative Stress/drug effects
- Pyrimidines/toxicity*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reactive Oxygen Species/metabolism
- Strobilurins/toxicity*
- Water Pollutants, Chemical/toxicity
- Zebrafish/anatomy & histology
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics
- PubMed
- 29529468 Full text @ Aquat. Toxicol.
Citation
Cao, F., Wu, P., Huang, L., Li, H., Qian, L., Pang, S., Qiu, L. (2018) Short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish (Danio rerio). Aquatic toxicology (Amsterdam, Netherlands). 198:129-140.
Abstract
Previous study indicated that azoxystrobin had high acute toxicity to zebrafish, and larval zebrafish were more sensitive to azoxystrobin than adult zebrafish. The objective of the present study was to investigate short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish. After zebrafish embryos and adults were exposed to 0.01, 0.05 and 0.20 mg/L azoxystrobin (equal to 25, 124 and 496 nM azoxystrobin, respectively) for 8 days, the lethal effect, physiological responses, liver histology, mitochondrial ultrastructure, and expression alteration of genes related to mitochondrial respiration, oxidative stress, cell apoptosis and innate immune response were determined. The results showed that there was no significant effect on larval and adult zebrafish after exposure to 0.01 mg/L azoxystrobin. However, increased ROS, MDA concentration and il1b in larval zebrafish, as well as increased il1b, il8 and cxcl-c1c in adult zebrafish were induced after exposure to 0.05 mg/L azoxystrobin. Reduced mitochondrial complex III activity and ATP concentration, increased SOD activity, ROS and MDA concentration, decreased cytb, as well as increased sod1, sod2, cat, il1b, il8 and cxcl-c1c were observed both in larval and adult zebrafish after exposure to 0.20 mg/L azoxystrobin; meanwhile, increased p53, bax, apaf1 and casp9, alteration of liver histology and mitochondrial ultrastructure in larval zebrafish, and alteration of mitochondrial ultrastructure in adult zebrafish were also induced. The results demonstrated that azoxytrobin induced short-term developmental effects on larval zebrafish and adult zebrafish, including mitochondrial dysfunction, oxidative stress, cell apoptosis and innate immune response. Statistical analysis indicated that azoxystrobin induced more negative effects on larval zebrafish, which might be the reason for the differences of developmental toxicity between larval and adult zebrafish caused by azoxystrobin. These results provided a new insight into potential mechanisms of azoxystrobin in larval zebrafish and adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping