ZFIN ID: ZDB-PUB-180310-1
The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
Dancevic, C.M., Gibert, Y., Berger, J., Smith, A.D., Liongue, C., Stupka, N., Ward, A.C., McCulloch, D.R.
Date: 2018
Source: International Journal of Molecular Sciences   19(3): (Journal)
Registered Authors: Berger, Joachim, Gibert, Yann, Liongue, Clifford, Ward, Alister
Keywords: ADAMTS, extracellular matrix, metalloproteinase, muscle, somite, zebrafish
MeSH Terms:
  • ADAMTS5 Protein/genetics*
  • ADAMTS5 Protein/metabolism
  • Animals
  • Cell Differentiation*
  • Embryo, Nonmammalian
  • Extracellular Space
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Hedgehog Proteins/metabolism
  • Morphogenesis/genetics
  • Muscle Development/genetics
  • Signal Transduction
  • Somites/embryology*
  • Somites/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 29518972 Full text @ Int. J. Mol. Sci.
The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.