PUBLICATION
The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation
- Authors
- Dancevic, C.M., Gibert, Y., Berger, J., Smith, A.D., Liongue, C., Stupka, N., Ward, A.C., McCulloch, D.R.
- ID
- ZDB-PUB-180310-1
- Date
- 2018
- Source
- International Journal of Molecular Sciences 19(3): (Journal)
- Registered Authors
- Berger, Joachim, Gibert, Yann, Liongue, Clifford, Ward, Alister
- Keywords
- ADAMTS, extracellular matrix, metalloproteinase, muscle, somite, zebrafish
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/genetics*
- Cell Differentiation*
- ADAMTS5 Protein/genetics*
- ADAMTS5 Protein/metabolism
- Muscle Development/genetics
- Gene Expression Regulation, Developmental
- Gene Silencing
- Embryo, Nonmammalian
- Animals
- Morphogenesis/genetics
- Hedgehog Proteins/metabolism
- Somites/embryology*
- Somites/metabolism*
- Signal Transduction
- Extracellular Space
- PubMed
- 29518972 Full text @ Int. J. Mol. Sci.
Citation
Dancevic, C.M., Gibert, Y., Berger, J., Smith, A.D., Liongue, C., Stupka, N., Ward, A.C., McCulloch, D.R. (2018) The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation. International Journal of Molecular Sciences. 19(3).
Abstract
The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping