PUBLICATION
Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos
- Authors
- Tsai, T.C., Shih, C.C., Chien, H.P., Yang, A.H., Lu, J.K., Lu, J.H.
- ID
- ZDB-PUB-180307-1
- Date
- 2018
- Source
- BMC Developmental Biology 18: 5 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Holt-Oram syndrome, Insulin-like growth factor I, Zebrafish, tbx5
- MeSH Terms
-
- Animals
- Apoptosis*/drug effects
- Apoptosis*/genetics
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Gene Expression Regulation, Developmental/drug effects
- Humans
- Insulin-Like Growth Factor I/pharmacology*
- Morphogenesis*/drug effects
- Morphogenesis*/genetics
- Morpholinos/pharmacology
- Myocardium/metabolism
- Myocytes, Cardiac/drug effects
- Myocytes, Cardiac/metabolism
- Phenotype
- Phosphorylation/drug effects
- Survival Analysis
- T-Box Domain Proteins/deficiency*
- T-Box Domain Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 29506474 Full text @ BMC Dev. Biol.
Citation
Tsai, T.C., Shih, C.C., Chien, H.P., Yang, A.H., Lu, J.K., Lu, J.H. (2018) Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos. BMC Developmental Biology. 18:5.
Abstract
Background Tbx5 deficiency in zebrafish causes several abnormal phenotypes of the heart and pectoral fins. It has been reported that exogenous human growth hormone can enhance expression of downstream mediators in the growth hormone and insulin-like growth factor I (IGF-I) pathway and partially restore dysmorphogenesis in tbx5 morphants. This study aimed to further evaluate the effects of IGF-I on cell apoptosis and dysmorphogenesis in zebrafish embryos deficient for tbx5.
Results Among the five studied groups of zebrafish embryos (wild-type embryos [WT], tbx5 morphants [MO], mismatched tbx5 morpholino-treated wild-type embryos [MIS], IGF-I-treated wild-type embryos [WTIGF1], and IGF-I-treated tbx5 morphants [MOIGF1]), the expression levels of the ifg1, igf1-ra, ifg-rb, erk1, and akt2 genes as well as the ERK and AKT proteins were significantly reduced in the MO group, but were partially restored in the MOIGF1 group. These expression levels remained normal in the WT, MIS, and WTIGF1 groups. Exogenous human IGF-I also reduced the incidence of phenotypic anomalies, decreased the expression levels of apoptotic genes and proteins, suppressed cell apoptosis, and improved survival of the MOIGF1 group.
Conclusions These results suggest that IGF-I has an anti-apoptotic protective effect in zebrafish embryos with tbx5 deficiency.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping