PUBLICATION
Developmental Toxicity of Diethylnitrosamine in Zebrafish Embryos/Juveniles Related to Excessive Oxidative Stress
- Authors
- Huang, D., Li, H., He, Q., Yuan, W., Chen, Z., Yang, H.
- ID
- ZDB-PUB-180306-2
- Date
- 2018
- Source
- Water, air, and soil pollution 229: 81 (Journal)
- Registered Authors
- Keywords
- Developmental toxicity, Diethylnitrosamine, Oxidative stress, Teratogenicity, Zebrafish
- MeSH Terms
- none
- PubMed
- 29503482 Full text @ Water Air Soil Pollut
Citation
Huang, D., Li, H., He, Q., Yuan, W., Chen, Z., Yang, H. (2018) Developmental Toxicity of Diethylnitrosamine in Zebrafish Embryos/Juveniles Related to Excessive Oxidative Stress. Water, air, and soil pollution. 229:81.
Abstract
Diethylnitrosamine (DEN) is present in food, water, and daily supplies and is regarded as a toxicant of carcinogenicity. The developmental toxicity of DEN has been rarely reported as yet. In this study, zebrafish were exposed to different concentrations of DEN at 6 h post-fertilization (hpf) to access embryonic toxicity of the compound. The results show that DEN resulted in negative effects of hatching rate, heartbeat, body length, and spontaneous movement. Deformities, including notochord malformation, pericardium edema, embryonic membrane turbidity, tail hypoplasia, yolk sac deformity, and growth retardation, happened during exposure period. Moreover, production of reactive oxygen species (ROS) significantly increased after DEN treatment. Then, alterations of the expression level of oxidative stress-related genes were observed in our results. To our knowledge, this is the first study concerning the effect of DEN on zebrafish. And from the information of our research, we speculated that development toxicity of DEN should be related to the excessive oxidative stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping