PUBLICATION
Time does matter! Acute copper exposure abolishes rhythmicity of clock gene in Danio rerio
- Authors
- Doria, H.B., Ferreira, M.B., Rodrigues, S.D., Lo, S.M., Domingues, C.E., Nakao, L.S., de Campos, S.X., Ribeiro, C.A.O., Randi, M.A.F.
- ID
- ZDB-PUB-180303-15
- Date
- 2018
- Source
- Ecotoxicology and environmental safety 155: 26-36 (Journal)
- Registered Authors
- Keywords
- Antioxidant enzymes, Circadian clock, Copper, Danio rerio
- MeSH Terms
-
- Animals
- Catalase/metabolism
- Circadian Clocks/drug effects*
- Copper/toxicity*
- Cryptochromes/genetics
- Cryptochromes/metabolism
- Eye Proteins/genetics
- Eye Proteins/metabolism
- Female
- Gene Expression/drug effects
- Male
- Period Circadian Proteins/genetics
- Period Circadian Proteins/metabolism*
- Superoxide Dismutase/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29499429 Full text @ Ecotoxicol. Environ. Saf.
Citation
Doria, H.B., Ferreira, M.B., Rodrigues, S.D., Lo, S.M., Domingues, C.E., Nakao, L.S., de Campos, S.X., Ribeiro, C.A.O., Randi, M.A.F. (2018) Time does matter! Acute copper exposure abolishes rhythmicity of clock gene in Danio rerio. Ecotoxicology and environmental safety. 155:26-36.
Abstract
The circadian clock is a key cellular timing system that coordinates physiology and behavior. Light is a key regulator of the clock mechanism via its activation of Per and Cry clock gene expression. Evidence points to a key role of reactive oxygen species (ROS) in resetting this process. In this context, the aim of the present study was to explore copper as a ROS generator, using an innovative approach investigating its effects on circadian timing. Liver and brain from Danio rerio specimens exposed to 0, 5, 25 and 45 μg/L copper concentrations were obtained. Daily oscillations of superoxide dismutase (SOD) and catalase (CAT) enzymatic activity and their correlations both with clock genes (per1, per2, and cry1a) and with organism energy cost were determined. CAT expression correlates with per2 and cry1a and, thus, provides data to support the hypothesis of hydrogen peroxide production by a phototransducing flavin-containing oxidase. Higher SOD activity is correlated with higher intracellular ATP levels. Copper disturbed the daily oscillation of antioxidant enzymes and clock genes, with disturbed per1 rhythmicity in both the brain and liver, while cry1a rhythmicity was abolished in the liver at 25 μg/L copper. Coordination between the SOD and the CAT enzymes was lost when copper concentrations exceeded the limits established by international laws. These results indicate that organism synchronization with the environment may be impaired due to acute copper exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping