PUBLICATION
Molecular cloning, characterization and expression profiling of galectin-9 gene from Labeo rohita (Hamilton, 1822)
- Authors
- Mushtaq, Z., Krishnan, R., Prasad, K.P., Bedekar, M.K., Kumar, A.P.
- ID
- ZDB-PUB-180227-3
- Date
- 2018
- Source
- Fish & shellfish immunology 76: 287-292 (Journal)
- Registered Authors
- Keywords
- A. hydrophila, Galectin-9, Gene expression, Labeo rohita, Pathogen recognition receptor, ?-galactoside
- MeSH Terms
-
- Gene Expression Profiling/veterinary
- Immunity, Innate/genetics*
- Aeromonas hydrophila/physiology
- Galectins/chemistry
- Galectins/genetics*
- Galectins/immunology*
- Base Sequence
- Phylogeny
- Fish Diseases/immunology*
- Sequence Alignment/veterinary
- Animals
- Cyprinidae/genetics*
- Cyprinidae/immunology*
- Gene Expression Regulation/immunology*
- Gram-Negative Bacterial Infections/immunology
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Amino Acid Sequence
- PubMed
- 29477496 Full text @ Fish Shellfish Immunol.
Citation
Mushtaq, Z., Krishnan, R., Prasad, K.P., Bedekar, M.K., Kumar, A.P. (2018) Molecular cloning, characterization and expression profiling of galectin-9 gene from Labeo rohita (Hamilton, 1822). Fish & shellfish immunology. 76:287-292.
Abstract
Galectin-9 is a b-galactoside-binding tandem repeat galectin that regulates many cellular functions, ranging from cell adhesion to pathogen recognition. In spite of extensive study of mammalian galectin importance in immune system, little is known about that of fish. To study the normal expression and immune response of Labeo rohita to pathogens, a tandem-repeat galectin-9 from Labeo rohita was identified and named LrGal-9. Its full-length cDNA was 1534 bp encoded 291 amino acids (35.12?KDa), shared the highest 81% identity with the galectin-9 of Danio rerio. LrGal-9 identified in this study lacked signal peptide and a transmembrane domain like galectin-9 members reported in other fishes. Quantitative PCR showed that LrGal-9 was lowly expressed in gill, muscle, heart, highly expressed in tested immune tissues (intestine, kidney, liver, spleen) in normal body. After Aeromonas hydrophila challenge, LrGal-9 was remarkably increased in all tested immune tissues in a time-dependent manner. These results suggest that LrGal-9 plays a role in innate immunity in Labeo rohita.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping