PUBLICATION

Spinal cholinergic interneurons differentially control motoneuron excitability and alter the locomotor network operational range

Authors
Bertuzzi, M., Ampatzis, K.
ID
ZDB-PUB-180202-4
Date
2018
Source
Scientific Reports   8: 1988 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Receptors, Muscarinic/metabolism
  • Zebrafish
  • Interneurons/physiology*
  • Spinal Cord/physiology*
  • Cholinergic Neurons/physiology*
  • Locomotion/physiology*
  • Models, Biological
  • Animals
  • Motor Neurons/physiology*
(all 9)
PubMed
29386582 Full text @ Sci. Rep.
Abstract
While cholinergic neuromodulation is important for locomotor circuit operation, the specific neuronal mechanisms that acetylcholine employs to regulate and fine-tune the speed of locomotion are largely unknown. Here, we show that cholinergic interneurons are present in the zebrafish spinal cord and differentially control the excitability of distinct classes of motoneurons (slow, intermediate and fast) in a muscarinic dependent manner. Moreover, we reveal that m2-type muscarinic acetylcholine receptors (mAChRs) are present in fast and intermediate motoneurons, but not in the slow motoneurons, and that their activation decreases neuronal firing. We also reveal a strong correlation between the muscarinic receptor configuration on motoneurons and the ability of the animals to locomote at different speeds, which might serve as a plasticity mechanism to alter the operational range of the locomotor networks. These unexpected findings provide new insights into the functional flexibility of motoneurons and how they execute locomotion at different speeds.
Genes / Markers
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-chatpolyclonalGoat
Ab1-slc18a3polyclonal
    Guinea pig
    Ab2-chrm2polyclonal
      Rabbit
      1 - 3 of 3
      Show
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available