PUBLICATION
Role of Quzhou Fructus Aurantii Extract in Preventing and Treating Acute Lung Injury and Inflammation
- Authors
- Li, L., Zhang, S., Xin, Y., Sun, J., Xie, F., Yang, L., Chen, Z., Chen, H., Liu, F., Xuan, Y., You, Z.
- ID
- ZDB-PUB-180128-1
- Date
- 2018
- Source
- Scientific Reports 8: 1698 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Acute Lung Injury/prevention & control*
- Animals
- Animals, Genetically Modified
- Anti-Inflammatory Agents/administration & dosage*
- Anti-Inflammatory Agents/isolation & purification
- Bronchoalveolar Lavage Fluid/cytology
- China
- Cytokines/analysis
- Disease Models, Animal
- Lung/pathology
- Macrophages/drug effects
- Mice
- Plant Extracts/administration & dosage*
- Plant Extracts/isolation & purification
- Plants, Medicinal/chemistry*
- Pneumonia/prevention & control*
- RAW 264.7 Cells
- Treatment Outcome
- Zebrafish
- PubMed
- 29374248 Full text @ Sci. Rep.
Citation
Li, L., Zhang, S., Xin, Y., Sun, J., Xie, F., Yang, L., Chen, Z., Chen, H., Liu, F., Xuan, Y., You, Z. (2018) Role of Quzhou Fructus Aurantii Extract in Preventing and Treating Acute Lung Injury and Inflammation. Scientific Reports. 8:1698.
Abstract
Quzhou Fructus Aurantii (QFA) is an authentic herb of local varieties in Zhejiang, China, which is usually used to treat gastrointestinal illnesses, but its effects on respiratory inflammation have not been reported yet. In our study, the anti-inflammatory activity of QFA extract (QFAE) was evaluated on copper sulfate pentahydrate (CuSO4·5H2O)-induced transgenic neutrophil fluorescent zebrafish model. QFAE showed a significant effect of anti-inflammation in CuSO4·5H2O-induced zebrafish by reducing the neutrophil number in the inflammatory site. We investigated the anti-inflammatory activity of QFAE on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice models and RAW 264.7 cells. QFAE had an anti-inflammatory effect on reducing total cells, neutrophils, and macrophages in BALF and attenuated alveolus collapse, neutrophils infiltration, lung W/D ratio, myeloperoxidase (MPO) protein expression and other pulmonary histological changes in lung tissues, as well as hematological changes. Levels of pro-inflammatory cytokines, including TNF, IL-6, IFN-γ, MCP-1, and IL-12p70, were decreased, whereas anti-inflammatory cytokine IL-10 was increased after treatment with QFAE both in vivo and in vitro. In summary, our results suggested that QFAE had apparent anti-inflammatory effects on CuSO4·5H2O-induced zebrafish, LPS-induced ALI mice, and RAW 264.7 cells. Furthermore, QFAE may be a therapeutic drug to treat ALI/ARDS and other respiratory inflammations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping