PUBLICATION
Sound shock response in larval zebrafish: A convenient and high-throughput assessment of auditory function
- Authors
- Liu, X., Lin, J., Zhang, Y., Guo, N., Li, Q.
- ID
- ZDB-PUB-180114-3
- Date
- 2018
- Source
- Neurotoxicology and teratology 66: 1-7 (Journal)
- Registered Authors
- Keywords
- Acoustic startle response, Broad-band noise, Hearing ability, Zebrafish larvae
- MeSH Terms
-
- Acoustic Stimulation
- Animals
- Gentamicins/toxicity
- Hair Cells, Auditory/drug effects
- Hearing/drug effects
- Hearing/physiology*
- Hearing Tests
- Larva/physiology*
- Lateral Line System/drug effects*
- Noise*
- Reflex, Startle/drug effects
- Reflex, Startle/physiology*
- Zebrafish/physiology*
- PubMed
- 29330026 Full text @ Neurotoxicol. Teratol.
Citation
Liu, X., Lin, J., Zhang, Y., Guo, N., Li, Q. (2018) Sound shock response in larval zebrafish: A convenient and high-throughput assessment of auditory function. Neurotoxicology and teratology. 66:1-7.
Abstract
Given that hearing ability can be challenged in diverse ways, it is necessary to develop an easily conducted, high-throughput method for assessing potential auditory risks. Measuring the acoustic startle response (ASR) has become a critical behavioral method in hearing research using zebrafish (Danio rerio). In this study, changes in the activity of zebrafish larvae (10 days post fertilization (dpf)) due to exposure to a sudden easily-generated broad-band noise were automatically and objectively recorded and analyzed without building sophisticated equipments. A significant increase in activity was induced by the noise stimulation and the alterations were impaired by gentamicin. In addition, a clear dose-response trend was observed between gentamicin exposure and the impaired activity, and a similar phenomenon was observed between gentamicin exposure and damage to hair cells. Our results suggested that alterations in the activity induced by a broad-band noise can potentially be used as an efficient assay for assessing hearing ability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping